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采用５个ＰｓｔⅠ／ＭｓｅⅠ引物组合对来自２个籼稻品种Ｈ３５９和Ａｃｃ８５５８的重组自交系（ＲＩ，Ｆ７代，１３１个系）群体进行了扩增片断长度多态性（ＡＦＬＰ）分析，并对ＡＦＬＰ的多态性程度、ＡＦＬＰ标记的染色体分布及分离比例进行了研究．在ＡＦＬＰ分析中，共检测到９８个多态性带，其中７８个定位到连锁图中，并分布在所有的１２条染色体上，表明ＡＦＬＰ是一种高效的分子标记，可与ＲＦＬＰ标记结合应用于遗传图谱的构建和基因定位．
Five PstⅠ / MseⅠ primer combinations were used to analyze the amplified fragment length polymorphism (AFLP) of two inbred lines (RI, F7 and 131 lines) from two indica rice varieties H359 and Acc8558. AFLP The degree of polymorphism, chromosome distribution of AFLP markers and the ratio of segregation were studied. In AFLP analysis, a total of 98 polymorphic bands were detected, of which 78 were mapped to the linkage map and distributed on all 12 chromosomes, indicating that AFLP is a highly efficient molecular marker that can be used in conjunction with RFLP markers Genetic map construction and gene mapping.