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利用实验室筛选的能够通过“4S”途径进行脱硫代谢的红平红球菌H-412(Rhodococcus sp.H-412)作为生物脱硫催化剂,二苯并噻吩(DBT)为含硫模型化合物,考察了红球菌H-412生长细胞脱除正十六烷中有机硫的脱硫性能。在油相体积分数20%的反应体系中,相同浓度的生长细胞比休止细胞的脱硫效率高。利用红球菌H-412生长细胞在油相分率20%的情况下,初始发酵液菌体浓度为0.048 g/L,初始DBT浓度为0.25 mmol/L的条件下分批培养的脱硫效率较高,达到了57%。红球菌H-412生长细胞在油相体积分率分别为0,5%,10%和20%条件下进行脱硫,DBT的消耗量及二羟基联苯(2-HBP)的生成量随油相体积增加而增大,20%条件下均达最大值,脱硫效率最高。细胞生长量随油相体积增大而减少,无油存在的条件下,细胞生长量最大。
Rhodococcus sp. H-412 (Rhodococcus sp. H-412), which can be desulphurized and metabolized through the “4S” pathway, was selected as a bio-desulfurization catalyst by lab. Dibenzothiophene (DBT) The desulfurization performance of organosulfur in n-hexadecane from Rhodococcus sp. H-412 growing cells was investigated. In the reaction system with 20% oil volume fraction, the same concentration of growing cells was more efficient than the resting cells. In the case of Rhodococcus sp.H-412, the desulfurization efficiency of batch culture was higher under the conditions of oil phase fraction of 20%, initial fermentation broth concentration of 0.048 g / L and initial DBT concentration of 0.25 mmol / L , Reached 57%. The growth of Rhodococcus sp. H-412 cells was desulfurized at the oil phase volume fractions of 0, 5%, 10% and 20%, respectively. The consumption of DBT and the formation of 2-HBP Volume increases and increases, reaching the maximum under 20%, the highest desulfurization efficiency. The amount of cell growth decreased with the increase of oil phase volume, and the cell growth was maximal under the condition of no oil.