Objective:The purpose of this study was to study the clinical and pathological characteristics of breast intraductal proliferative lesions (IDPLs) and associated with invasive breast cancer. Methods: We reviewed 327 cases of breast intra-ductal proliferative lesions including 53 cases of usual ductal hyperplasia, 57 cases of atypical ductal hyperplasia, 89 cases of ductal carcinoma in situ, and 128 cases coexist with invasive ductal carcinomas. Cases of pure invasive cancer without intraductal proliferative lesions were excluded. The mult IDPLs biological parameters including the express of ER, PR, HER2, HIF-1αand Ki-67 detected by immunohistochemistry S-P method (n=327) and the levels of CA153, TSGF, CA125 and CEA both in nipple discharge and serum (n=179) measured with Electrochemiluminescence method and their relationship were studied, and 30 cases of normal pregnant women were compared with. Results: A single histologic subtype was present in 49.85%(163/327) of the cases, two subtypes in 33.03%(108/327), and three in 17.13%(56/327). The most common subtypes present were cribriform (43.12%, 141/327) and solid (38.53%, 126/327), while the comedo (16.35%, 54/327), and micropapil ary (12.84%, 42/327) subtypes were less common. Comedo and solid were frequently found together for coexpres-sion as were micropapil ary and papil ary subtypes. However, Comedo subtype was much less likely to be found with papil ary, cribriform or micropapil ary subtypes. Additional y, comedo subtypes tend to be hormone receptor negative, Her2 positive and high-grade while the cribriform and solid subtype tends to be hormone receptor positive, Her2 negative and low grade. Papil-lary subtype was least likely to be associated with an invasive cancer. Furthermore, the nipple discharge and serum levels of CA153, TSGF, CA125 and CEA in coexist with invasive ductal carcinomas patients were significantly higher than those in the benign breast disease (pure intraductal proliferative lesions) and normal pregnant women (P