Objective:The aim of the study was to explore the role of Wnt/β-catenin signal ing pathway in the maintenance, invasion and metastasis of colorectal cancer stem cells. Methods:Double immunohistochemical staining was used to detect the expression of EpCAMhigh/CD44+which is regarded as the marker of colorectal cancer stem cells in 80 cases of colorectal cancer and their corresponding liver metastases. The SP method of immunohistochemistry was used to detect the expression of the key proteinβ-catenin in the Wnt pathway in these tissue. The expression and correlation ofβ-catenin and EpCAMhigh/CD44+in colorectal cancer were analyzed and their role on the biological behavior of colorectal cancer was explored. Results:The abnormal expression ofβ-catenin was significantly higher in colorectal cancer than in the paraneoplastic normal intes-tinal mucosa [55%(44/80) vs 10%(2/20), P0.05), but was significantly correlated with age (P=0.021), degree of dif erentiation (P=0.013), depth of invasion (P=0.000), clinical stage (P=0.000) and metastasis (P=0.000). In the corresponding liver metastases, we could also detecte EpCAMhigh/CD44+cells. In cases with abnormal expression ofβ-catenin, the positive expression rate of EpCAMhigh/CD44+was significantly higher than those with normal expression ofβ-catenin (84.1%vs 44.4%), and the dif erence was statistical y significant (P