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目的 寻找系统性红斑狼疮中诱导抗核抗体 (ANA)生成的真正的自身核成分免疫原。方法 从ConA活化的脾淋巴细胞中提取组蛋白免疫同系BALB/c小鼠 ,ELISA方法测定IgG类抗组蛋白、抗dsDNA抗体 ,免疫荧光法检测抗核抗体核型和免疫复合物在肾小球内的沉积 ,免疫印迹法测定抗可溶性核抗原抗体 ,考马斯亮蓝法检测尿蛋白含量 ,光镜下观察肾脏形态变化 ,电镜下观察肾小球电子致密物。结果 活性组蛋白诱导IgG类抗组蛋白、抗dsDNA等多种抗核抗体生成 ,且诱发同系小鼠产生SLE样肾脏病理变化。结论 活性组蛋白是诱发抗核抗体生成、造成肾损害的免疫原之一
Objective To search for a true self-nuclear component immunogen induced by anti-nuclear antibody (ANA) in systemic lupus erythematosus (SLE). METHODS: Histone immunocompetent BALB / c mice were isolated from ConA-activated splenic lymphocytes and IgG anti-histone and anti-dsDNA antibodies were determined by ELISA. The karyotype of anti-nuclear antibody and immune complexes were detected by immunofluorescence in glomeruli The anti-soluble nuclear antigen antibody was detected by immunoblotting. Urine protein content was detected by Coomassie Brilliant Blue method. The morphological changes of kidney were observed under light microscope. Electron microscopy was used to observe the electron density of glomeruli. Results The active histones induced many anti-nuclear antibodies such as IgG anti-histone and anti-dsDNA, and induced the pathological changes of SLE-like kidneys in syngeneic mice. Conclusions Active histones are one of the immunogens that induce the generation of anti-nuclear antibodies and cause renal damage