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背景:宫颈癌是危害女性身体健康,降低生活质量的疾病之一。利用中药治疗宫颈癌,降低放射治疗、应用抗肿瘤药物治疗副作用对机体伤害已成为医药界的研究热点。在欧洲,水飞蓟素曾广泛用于临床的肝炎治疗。它毒性小,在植物中含量高。因此,一些学者正在研究其抗肿瘤的活性。目的:探讨水飞蓟素对人子宫颈癌细胞的药理作用机制。设计:以体外10%血清正常培养的细胞作为阴性对照的实验对照研究。单位:沈阳药科大学中日医药研究所,昭和药科大学病态科学教研室。材料:人的宫颈癌HeLa细胞为本实验室保存的美国ATCC细胞系。实验于2003-02/07在沈阳药科大学中日医药研究所细胞室完成。方法:噻唑蓝还原法(MTT)分析HeLa细胞的死亡率。采用相差和荧光显微镜观察细胞形态学改变。用流式细胞术分析了细胞周期的变化。主要观察指标:细胞死亡率和细胞周期分化的变化。结果:水飞蓟素诱导的HeLa细胞死亡与培养液中血清含量有关。给予80μmol/L的水飞蓟素作用,去血清组的细胞死亡率达到85.27%,含5%,10%血清组的死亡率为35.53%,7.71%。细胞的形态学变化表明水飞蓟素诱导的HeLa细胞发生凋亡。流式细胞分析结果显示:水飞蓟素诱导70.04%的细胞发生凋亡,这些凋亡细胞主要来自G0/G1期。结论:水飞蓟素在去血清条件下,诱导处于G0/G1期的
Background: Cervical cancer is one of the diseases endangering women’s health and reducing the quality of life. The use of traditional Chinese medicine treatment of cervical cancer, reduce radiation therapy, the application of anti-tumor drugs to treat side effects on the body injury has become a medical research hotspot. In Europe, silymarin has been widely used in clinical treatment of hepatitis. It is less toxic and high in plants. Therefore, some scholars are studying its anti-tumor activity. Objective: To investigate the pharmacological mechanism of silymarin on human cervical cancer cells. DESIGN: An experimental controlled study of cells normally cultured in vitro with 10% serum as a negative control. Unit: Shenyang Pharmaceutical University, Japan Institute of Medicine, Showa Pharmaceutical University, Department of Pathology. MATERIALS: Human cervical cancer HeLa cells were deposited in our laboratory with the American ATCC cell line. The experiment was performed in the cell room of Institute of Chinese Medicine, Shenyang Pharmaceutical University from February to July in 2003. Methods: The mortality of HeLa cells was analyzed by MTT assay. Morphological changes were observed by phase contrast and fluorescence microscopy. Cell cycle changes were analyzed by flow cytometry. MAIN OUTCOME MEASURES: Changes in cell death rate and cell cycle differentiation. Results: The silymarin-induced HeLa cell death was related to the serum content in culture medium. Given the effect of silymarin at a concentration of 80 μmol / L, the cell death rate reached 85.27% with 5% in the serum-free group and 35.53% with 7.71% in the 10% serum group. Morphological changes of the cells indicate that silymarin-induced HeLa cells undergo apoptosis. Flow cytometry analysis showed that silymarin induced apoptosis in 70.04% of cells, and these apoptotic cells mainly came from G0 / G1 phase. Conclusion: Silymarin induces G0 / G1 phase under serum-free conditions