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前期研究从杨柳田头菇(Agrocybe salicacola)YAASM0711的单核菌株M20中获得了含有GC-AG内含子的3-磷酸甘油醛脱氢酶(Glycerol-3-phosphate dehydrogenase,GPD)gpd完整编码基因,本研究通过特异性引物PCR扩增探讨该基因的组成结构及内含子的拼接特征.在杨柳田头菇和茶树菇(A.aegerita)野生群体中均获得了此基因,序列差异性分析显示该基因可被分为两类,并且在两个物种中均有分布,表明这种序列组成差异可能在两个物种分化之前就已经存在.另外,在M20菌株的菌丝体及YAASM0711菌株的不同发育时期均没有发现可选择性剪切现象.基因表达分析表明,gpd在幼嫩的子实体中表达量最高,约为菌丝体中表达量的5.5倍,推测与子实体的快速生长有关.本研究结果有助于提高对gpd功能及其在物种分化研究中作用的认识.
Previous studies obtained the gpd complete coding gene of glyceral-3-phosphate dehydrogenase (GPD) containing GC-AG intron from the monokaryotic strain M20 of Agrocybe salicacola YAASM0711 In this study, we investigated the composition of the gene and the intron mosaic features by PCR amplification of specific primers.This gene was obtained from wild populations of A. yunnanensis and A. aegerita, and sequence diversity analysis This gene was shown to be divided into two groups and distributed in both species, indicating that the differences in sequence composition may have existed prior to the differentiation of both species.In addition, in the mycelium of M20 strain and in the strain of YAASM0711 At different developmental stages, no selective cleavage was observed.Gene expression analysis showed that gpd was the highest expression in young fruiting bodies, about 5.5 times that in mycelium, suggesting that it was related to the rapid growth of fruiting bodies The results of this study contribute to raising awareness of gpd function and its role in the study of species differentiation.