目的研究金水鲜联合放疗对肺腺癌A549细胞实验处理后的细胞存活力,以及抗血管生成蛋白表达与凋亡相关蛋白表达的关系变化。方法将生长状态良好的A549细胞按处理因素的不同分为4组,生理盐水(NC)组、金水鲜(JSX)组、放射治疗+金水鲜(RT+JSX)组、放射治疗(RT)组,用CCK8试剂盒处理4 h后酶标仪测OD450值,收集细胞裂解后测定蛋白浓度并蛋白凝胶(WB)电泳,分析血管促进因子蛋白血管生成因子(VEGF)/胎盘源性生长因子(PIGF)和突变型P53以及促凋亡蛋白FAS蛋白的表达变化。结果①CCK8检测各组经过处理后细胞的增殖活力,RT+JSX组细胞的增殖活力最低,与JSX组比较,差异有高度统计学意义(P<0.01);JSX组与NC组比较,差异有统计学意义(P<0.05)。②Western-blot结果显示在第48小时,JSX组VEGF蛋白的表达下降明显;而在RT+JSX组中促凋亡蛋白FAS的表达明显增加,突变型P53蛋白的表达下降,与JSX组比较,差异有高度统计学意义(P<0.01),提示联合使用金水鲜增强了放疗自身的促凋亡作用。结论金水鲜联合放疗可能发挥药物单独具有的血管生成抑制作用,并能增强单用放疗的细胞促凋亡效果,考虑为金水鲜在影响血管生成方面能增加放疗的敏感性,激活细胞凋亡相关因子FAS和下调突变型P53,具体作用机制有待进一步证实。 Objective To study the changes of cell viability after treated with the combination of radix salviae miltiorrhizae and radiotherapy on lung adenocarcinoma A549 cells and the relationship between the expression of anti-angiogenic proteins and the expression of apoptosis-related proteins. Methods The A549 cells with good growth status were divided into four groups according to different treatment factors: normal saline (NC) group, JSX group, RT + JSX group, radiotherapy group , The OD450 value was measured by microplate reader after 4 h treatment with CCK8 kit, and the protein concentration and protein gel (WB) were measured after cell lysis. The expression of angiogenic factor VEGF / placenta-derived growth factor PIGF) and mutant P53 as well as the pro-apoptotic protein FAS protein expression changes. Results ①CCK8 detected the proliferative activity of cells in each group, and the activity of cells in RT + JSX group was the lowest. Compared with JSX group, the difference was statistically significant (P <0.01); there was statistical difference between JSX group and NC group Significance (P <0.05). ② Western-blot results showed that the expression of VEGF protein in JSX group decreased significantly at the 48th hour, while the expression of the pro-apoptotic protein FAS in RT + JSX group was significantly increased and the expression of the mutant P53 protein was decreased. Compared with JSX group, There was a high degree of statistical significance (P <0.01), suggesting that combined use of gold and water to enhance the role of radiotherapy itself to promote apoptosis. Conclusions The combination of Jinshuiqian and radiotherapy may exert the angiogenesis inhibitory effect of drugs alone and enhance the apoptosis promoting effect of radiotherapy alone. It is considered that Jinshuiqian can increase the sensitivity of radiotherapy in affecting angiogenesis and activate apoptosis Factor FAS and down-regulated mutant P53, the specific mechanism of action to be further confirmed.