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利用鲁麦10号小麦品种幼穗作为外植体诱导愈伤组织。研究了NaCl及γ射线对小麦愈伤组织的生长、脯氨酸含量及细胞形态大小的影响。结果如下:1.γ射线及NaCl都明显的抑制愈伤组织的生长。0.3%的NaCl和5KR的照射量是愈伤组织生长的临界值;30KR是致死的起始照射量:2.NaCl对愈伤组织的脯氨酸含量也有显著的影响,二者呈抛物线相关。γ射线使脯氨酸含量升高,但超过5KR,脯氨酸含量又呈下降的趋势:3.γ射线处理愈伤组织能使细胞的长及宽都增大,并产生巨型细胞,加速愈伤组织的老化。NaCl对愈伤组织细胞大小无显著影响,但能引起细胞大小变异系数的增加。
The young calli of Lumai 10 were used as explants to induce callus. The effects of NaCl and γ-rays on the growth, proline content and cell morphology of wheat callus were studied. The results are as follows: 1. Both γ-rays and NaCl significantly inhibited the growth of callus. 0.3% NaCl and 5KR irradiation is the critical value of callus growth; 30KR is the lethal initial exposure dose: 2.NaCl also has a significant effect on the proline content of callus, the two are parabolic. γ-ray proline content increased, but more than 5KR, proline content also showed a downward trend: 3.γ-ray treatment of callus cells can make the length and width are enlarged, and produce giant cells, accelerate the more Worn tissue aging. NaCl had no significant effect on the cell size of callus, but increased the coefficient of variation of cell size.