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目的 :探讨血清总胆汁酸(TBA)作为黄药子醇提物致肝损伤早期敏感标志物的可能性。方法:取Wistar大鼠随机分为对照组和黄药子醇提物组(以下简称黄药子组),黄药子组每天灌胃给予醇提物5 g/kg,对照组灌服蒸馏水,于给药后1 d、7 d、16 d分批处理动物。乌拉坦麻醉动物,取血,测定常规血液生化指标-丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TB)、碱性磷酸酶(ALP)、总蛋白(TP)、白蛋白(ALB)和文献报道的血清早期敏感指标-总胆汁酸(TBA)的水平,取肝,称重,计算肝脏指数并进行HE染色观察肝组织病理形态学变化。结果:与同时间点对照组相比,黄药子组常规生化指标AST和TB的变化较为明显,AST于给药1d～7d升高(P<0.01),TB于给药7 d有升高的趋势(P>0.05),16 d升高(P<0.01);肝脏指数于给药7d～16d升高(P<0.05或0.01),肝组织病理形态学检查显示16 d开始出现细胞肿大和单细胞坏死。血清早期敏感指标TBA于给药1d、7d、16d升高(P<0.05或0.01),与AST比较,TBA表现出良好的时效关系,较TB变化更早;与同时间点对照组相比,TBA数值的变化倍数均高于AST和TB。结论:TBA可作为黄药子致肝损伤的早期敏感生物标志物之一。
Objective: To investigate the possibility of serum total bile acid (TBA) as an early marker of hepatic injury induced by ethanol extract of Radix et Rhizoma Dioscoreae. Methods: The Wistar rats were randomly divided into control group and xanthium alcohol extract group (hereinafter referred to as xanthan group). The xanthate group was given gastric gavage daily with 5 g / kg ethanol, and the control group was given distilled water. After 1 d Animals were treated in batches on days 7 and 16. The animals were anesthetized with urethane and the blood was taken for determination of the common blood biochemical indicators - alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TB), alkaline phosphatase (ALP) , Total protein (TP), albumin (ALB), and serum total bile acid (TBA), respectively. The liver tissues were weighed, the liver index was calculated, and HE staining was performed to observe the pathological changes of liver tissue . Results: Compared with the control group at the same time point, the changes of routine biochemical indexes AST and TB in Huangyanzi group were more obvious. AST was increased from 1d to 7d after administration (P <0.01), TB increased on the 7th day after administration (P <0.05), and increased on the 16th day (P <0.01). The liver index increased from the 7th day to the 16th day after administration (P <0.05 or 0.01). The histopathological examination of liver tissue showed that the cell enlargement and single cell Necrosis. Compared with AST, TBA showed a good time-effect relationship, which was earlier than that of TB. Compared with the control group at the same time point, TBA, a sensitive index of early serum, increased at 1d, 7d and 16d (P <0.05 or 0.01) TBA values were higher than the fold change of AST and TB. Conclusion: TBA can be used as one of the early sensitive biomarkers of hepatic injury induced by xanthium.