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目的:观察槟榔十三味丸(高尤-13)对慢性应激抑郁模型大鼠行为学及海马神经元凋亡的影响,以期从细胞水平探讨该方抗抑郁症作用机制。方法:将48只Wistar雄性大鼠,根据蔗糖水消耗量及体质量随机分为正常对照组,模型组,氟西汀组(3.3 mg·kg-1),槟榔十三味丸低剂量组、中剂量组、高剂量组(0.25 g·kg-1,0.5 g·kg-1,1.0 g·kg-1),共6组,每组8只。除正常对照组外,其余大鼠均采用慢性轻度不可预见性应激结合孤养方法制备抑郁模型,造模同时灌胃给药,每日1次,连续给药28 d。以敞箱实验、蔗糖水消耗实验进行行为学评价,采用膜联蛋白V-异硫氰酸荧光素/碘化丙啶双标记(AnnexinVFITC/PI),以流式细胞仪(FC)检测海马神经元凋亡率。结果:与正常对照组比较,模型组大鼠水平运动及垂直运动得分、体质量、蔗糖水消耗量显著降低(P<0.01);与模型组比较,氟西汀组,槟榔十三味丸中剂量组、高剂量组大鼠水平运动及垂直运动得分、糖水消耗量、体质量均显著增加(P<0.05);流式细胞仪检测凋亡率显示,与正常对照组比较,模型组大鼠海马神经元凋亡率显著增高(P<0.05);与模型组比较,氟西汀组,槟榔十三味丸中剂量组、高剂量组大鼠海马神经元凋亡率降低,差异有显著性(P<0.01)。结论:慢性应激抑郁模型大鼠存在行为学异常及海马神经元凋亡现象,槟榔十三味丸能改善抑郁模型大鼠行为学异常及抑制慢性应激造成的大鼠海马神经元凋亡而发挥抗抑郁作用。
OBJECTIVE: To observe the effects of arecajianshiweiweiwan (Ganyu-13) on the behavior and the apoptosis of hippocampal neurons in rats with chronic stress depression, in order to explore the antidepressant mechanism at the cellular level. Methods: Forty-eight male Wistar rats were randomly divided into normal control group, model group, fluoxetine group (3.3 mg · kg-1), low-dose betel nut decoction group, Medium dose group and high dose group (0.25 g · kg -1, 0.5 g · kg -1, 1.0 g · kg -1), a total of 6 groups, 8 in each group. Except for the normal control group, the rest of the rats were treated with mild mild unpredictable stress combined with solitary method to make depression model. The model was given intragastrically once a day for 28 days. The behavior of hippocampal neurons was detected by flow cytometry (FC) using Annexin VFITC / PI with open-cell assay and sucrose water consumption test. Apoptosis rate. Results: Compared with the normal control group, the level of exercise and vertical movement scores, body weight and sucrose water consumption of the model group were significantly decreased (P <0.01). Compared with the model group, fluoxetine group, Compared with the normal control group, the rats in the model group (P <0.05) showed that the apoptosis rate of rats in the dose group and the high-dose group were significantly higher than those in the normal control group Hippocampal neurons apoptosis rate was significantly higher (P <0.05); Compared with the model group, fluoxetine group, arecajisanwei pill medium dose group, high dose group of hippocampal neuron apoptosis rate decreased, the difference was significant (P <0.01). CONCLUSION: Behavioral abnormalities and apoptosis of hippocampal neurons exist in chronic stress depression model rats. Arecajitang pill can improve behavioral abnormalities and inhibit the apoptosis of hippocampal neurons induced by chronic stress in rats with depression model Play an antidepressant effect.