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目的:研究4周低氧运动中mTOR/p70S6K通路的变化,以探讨低氧训练中的蛋白合成代谢情况。方法:以12周龄雄性SD大鼠为研究对象,分为常氧安静组(NS组)、常氧运动组(NE组)、低氧安静组(HS组)和低氧运动组(HE组)。采用动物跑台训练,低氧刺激为常压低氧(氧浓度13.6%,相当于3 500 m海拔)。检测大鼠趾长伸肌的总蛋白含量及mTOR、p70S6K和p70S6K(Thr389)磷酸化表达。结果:NE组和HE组mTOR蛋白表达均显著高于HS组。NE组p70S6K蛋白表达显著高于NS组和HS组。NE组p70S6K(Thr389)磷酸化显著高于HS组和HE组,NS组显著高于HS组和HE组,HE组显著高于HS组。结论:1)低氧抑制肌肉蛋白合成,常氧运动促进肌肉蛋白合成。2)长期低氧暴露抑制mTOR/p70S6K表达,运动促进表达,低氧运动削弱了低氧对mTOR/p70S6K表达的抑制作用。
Objective: To investigate the changes of mTOR / p70S6K pathway in hypoxia for 4 weeks to investigate the protein synthesis and metabolism in hypoxia training. Methods: Male Sprague-Dawley rats, 12 weeks old, were randomly divided into two groups: normoxia group (NS group), normoxia group (NE group), hypoxia quiescent group (HS group) and hypoxic exercise group ). Using animal treadmill training, hypoxia was normobaric hypoxia (oxygen concentration 13.6%, equivalent to 3 500 m elevation). The total protein content and the phosphorylation of mTOR, p70S6K and p70S6K (Thr389) in rat extensor digitorum longus muscle were measured. Results: The expressions of mTOR protein in NE group and HE group were significantly higher than those in HS group. The protein expression of p70S6K in NE group was significantly higher than that in NS group and HS group. The phosphorylation of p70S6K (Thr389) in NE group was significantly higher than that in HS group and HE group, while NS group was significantly higher than HS group and HE group. HE group was significantly higher than HS group. Conclusion: 1) hypoxia inhibits muscle protein synthesis, nocturnal exercise promote muscle protein synthesis. 2) Long-term hypoxia exposure inhibited the expression of mTOR / p70S6K and promoted the expression of mTOR / p70S6K. Hypoxia reduced the inhibition of mTOR / p70S6K expression.