论文部分内容阅读
目的检查腭、肺及鼻咽上皮克隆(PLUNC)基因编码区单核苷酸多态(SNP)位点G14595T 与广东地区鼻咽癌易感性有无明显关联。方法用直接测序的方法检测基因编码区、调控区和侧翼区及部分内含子区,确定 SNP 位置和类型。用聚合酶链式反应-限制性酶切片段长度多态性(PCR-RFLP)结合测序方法对编码区多态性位点 G14595T 在广东地区239例鼻咽癌患者和286例对照人群中进行相关性分析。结果通过测序共获取9个 SNP;其中8个 SNP 位点之间均呈高度连锁不平衡;3个 SNP 位点可以作为 htSNP;编码区多态位点 G14595T 在239例鼻咽癌患者和286例对照人群中等位基因型频率差别很小。结论 PLUNC 基因编码区多态位点 G14595T 在鼻咽癌患者和对照人群中等位基因型频率差别很小,这说明该位点与中国广东地区鼻咽癌易感性无明显关联。
Objective To investigate whether there is a clear correlation between the single nucleotide polymorphism (SNP) G14595T in the coding region of palate, lung and nasopharyngeal epithelial clonality (PLUNC) gene and susceptibility to nasopharyngeal carcinoma in Guangdong. Methods The gene coding region, regulatory region, flanking region and intron region were detected by direct sequencing and the location and type of SNP were determined. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with sequencing method was used to detect the polymorphism site G14595T of coding region in 239 nasopharyngeal carcinoma patients and 286 control subjects in Guangdong Sexual analysis. Results A total of 9 SNPs were obtained by sequencing. Among them, 8 SNP loci showed highly linkage disequilibrium; 3 SNP loci could serve as htSNP; G14595T coding region polymorphism loci in 239 patients with nasopharyngeal carcinoma and 286 patients The frequency of alleles in the control population is very small. Conclusion The allele frequency of G14595T, a polymorphism site in PLUNC gene coding region, is very small in patients with nasopharyngeal carcinoma and controls, indicating that this locus has no obvious correlation with susceptibility to nasopharyngeal carcinoma in Guangdong of China.