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以CB—HRP为标记物,分别用硝普钠(SNP)和庚钼酸铵(AHM)作四甲基联苯胺(TMB)反应的稳定剂,再用改进的Aldes平板包埋法(玻璃纸平板包埋法)包埋切片,对树鼩颈髓前角运动神经元作了光镜和电镜的比较观察。发现在光镜水平,TMB—SNP和TMB—AHM这两种显色反应对显示神经元的胞体和近端树突无明显差别。但在显示神经元的远端树突,尤其是白质树突方面,TMB—SNP反应明显优于TMB—AHM反应。而在电镜下,以AHM为稳定剂的标本,神经元间的突触、神经微丝、线粒体等亚细胞结构清楚;以SNP为稳定剂者,这些亚细胞结构,甚至神经细胞都难以辨认。改进的Aldes平板包埋法的电镜效果与原Aldes平板包埋法一致或更好,而方法更简便。
CB-HRP was used as stabilizer for tetramethylbenzidine (TMB) reaction with sodium nitroprusside (SNP) and ammonium heptamolybdate (AHM), respectively, and then modified with Aldes plate embedding method Embedding) embedded sections of the anterior horn of the tree neck spinal cord motor neurons made by light and electron microscopy comparison. It was found that at the light microscope level, the two chromogenic reactions of TMB-SNP and TMB-AHM showed no significant difference between the somatic and proximal dendrites of neurons. However, the TMB-SNP response was significantly superior to the TMB-AHM response in displaying distal dendrites of neurons, especially white matter dendrites. Under electron microscope, AHM as a stabilizer, synapses between neurons, neurofilaments, mitochondria and other subcellular structures clear; SNP as a stabilizer, these subcellular structures, and even nerve cells are difficult to identify. The improved Aldes plate embedding method electron microscopy effect with the original Aldes plate embedding method or better, and the method is more convenient.