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目的:观察缰核损毁及催眠药唑吡坦对视前区-下丘脑前部(PO/AH)腺苷和5-羟色胺(5-HT)相关基因mRNA表达的影响,探讨缰核在PO/AH促睡眠调节中的作用。方法:以30只雄性Wistar大鼠为实验动物,直流电损毁缰核或灌胃给予催眠药唑吡坦建立缰核损毁大鼠(n=15)和唑吡坦处理大鼠(n=15)两种动物模型,随后随机将大鼠分为缰核假手术组(n=7)、缰核损毁组(n=8)、生理盐水对照组(n=7)和唑吡坦处理组(n=8)。采用逆转录聚合酶链反应(RT-PCR)方法检测各组大鼠PO/AH内腺苷和5-HT相关基因mRNA的表达水平。结果:与假手术组相比,缰核损毁组大鼠PO/AH内除腺苷激酶mRNA表达的水平轻微升高(P>0.05)外,腺苷A1受体及5-HT相关基因mRNA的表达水平均明显上调(P<0.05)。与生理盐水对照组相比,唑吡坦处理组大鼠PO/AH内的5-HT相关基因mRNA表达明显降低(P<0.05);腺苷激酶的表达虽下调但无显著性差异(P>0.05),而腺苷A1受体的表达仅呈现轻微的上调(P>0.05)。结论:缰核对PO/AH的促睡眠过程具有调节作用,并且是通过腺苷和5-HT途径在此过程中发挥其作用。
Objective: To observe the effects of habenotomy nucleus damage and hypnotics zolpidem on the expression of adenosine and serotonin (5-HT) related genes in preoptic area-hypothalamus anterior part (PO / AH) AH to promote sleep regulation role. Methods: Thirty male Wistar rats were used as experimental animals. The habenula were destroyed by hamster or zolpidem by gavage. The rats in hantring failure (n = 15) and zolpidem (n = 15) The animals were randomly divided into hamstring hamstring injury group (n = 7), halotus injury group (n = 8), saline control group (n = 7) and zolpidem treatment group (n = 8). Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of adenosine and 5-HT related genes in PO / AH rats. Results: Compared with the sham operation group, the levels of adenosine kinase mRNA in PO / AH increased slightly (P> 0.05) in the habenular nucleus rupture group, while the expression of adenosine A1 receptor and 5-HT related gene mRNA The expression levels were significantly increased (P <0.05). Compared with the saline control group, the mRNA expression of 5-HT related gene in PO / AH of Zolpidem-treated group was significantly decreased (P <0.05), while the expression of adenosine kinase was not significantly different (P> 0.05), while adenosine A1 receptor expression showed only a slight increase (P> 0.05). CONCLUSION: The habenular nuclei have a regulatory effect on PO / AH promoting sleep and play its role in this process through adenosine and 5-HT.