昆虫病毒制剂在推广使用之前,必须对其标准品进行生物测定,确定其毒力,计算其有效成分含量,为制剂的标准化、工业化生产和大田应用提供科学依据。1982—1983年我们对本所研制的黄地老虎颗粒体病毒标准品及制剂进行了生物测定,兹简介于下。 一、材料与方法 供试幼虫为本室人工饲养的黄地老虎2龄末3龄初幼虫。幼虫平均体重4.5毫克,体长5—6毫米。 Before the promotion of the use of insect virus preparations, the bioassay of its standard products must be carried out to determine its virulence and calculate the content of its active ingredients so as to provide a scientific basis for the standardization of preparations, industrialized production and field application. From 1982 to 1983, we conducted a bioassay of the yellow tiger granulovirus standard and its formulation developed by our institute. I. Materials and Methods The larvae of the 3rd instar larvae at the end of the 2nd instar of the Yellow Tigers, which are artificially reared in this lab, Larva average body weight of 4.5 mg, body length 5-6 mm.