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目的 探讨山东沿海地区自身免疫性甲状腺病 (AITDs)与HLA DQA1 0 30 1、DR9的相关性。方法 采用多聚酶链式反应序列特异引物分析 (PCR SSP)技术 ,扩增HLA等位基因DQA1 0 30 1、DR9的目的DNA片段 (分别为 199、2 36bp) ,分析 2对等位基因在不同人群中表达频率的差异 (χ2 检验 )。结果 山东沿海地区GD和HT女性患者组DQA1 0 30 1等位基因频率均显著高于对照组 (分别为P <0 .0 0 1,OR =4 .89;P <0 .0 1,OR =4 .95 ) ;DR9等位基因频率仅HT女性组显著高于对照女性组(P <0 .0 5 ,OR =3.90 )。DQA1 0 30 1/DR9共同表达的频率 ,GD和HT女性组较对照女性组均显著性增高 (分别为P <0 .0 5 ,P <0 .0 1)。GD组和HT组 2组间均无显著性差异 (P >0 .0 5 )。结论 HLA DQA1 0 30 1等位基因是山东沿海地区女性GD患者的易感基因 ;DQA1 0 30 1、DR9等位基因均是该地区女性HT患者的易感基因 ;2对等位基因在男性AITDs患者中的分布情况尚待进一步观察
Objective To investigate the relationship between autoimmune thyroid disease (AITDs) and HLA DQA1 0 30 1, DR9 in Shandong coastal areas. Methods PCR-based polymerase chain reaction-specific primer analysis (PCR SSP) was used to amplify the DNA fragments of HLA alleles DQA1 0 30 1 and DR9 (199 and 236 bp, respectively). Two pairs of alleles were analyzed in different populations In the frequency of expression differences (χ2 test). Results The frequencies of DQA1 0 30 1 alleles in GD and HT female patients in Shandong coastal areas were significantly higher than those in control subjects (P <0.01, OR = 4.89, P <0.01, OR = 4.95). The frequency of DR9 allele in HT women was significantly higher than that in control women (P <0.05, OR = 3.90). The frequencies of DQA1 0 30 1 / DR9 co-expression were significantly higher in GD and HT women than in control women (P <0.05, P <0.01, respectively). There was no significant difference between GD group and HT group (P> 0.05). Conclusion The allele HLA DQA1 0 30 1 is a predisposing gene for female patients with GD in coastal areas of Shandong Province. The alleles of DQA1 0 30 1 and DR9 are the susceptible genes in female HT patients in this area. The alleles of two alleles in male patients with AITDs The distribution in patients remains to be seen