论文部分内容阅读
【目的】探讨不同免疫途径沙眼衣原体(Chlamydia trachomatis,Ct)分泌性蛋白Pgp3的免疫保护效果,分析其可能的保护机制,以确定Pgp3蛋白疫苗在Ct疫苗研制中的应用价值。【方法】分泌性蛋白Pgp3经滴鼻或肌注途径免疫雌性Balb/c小鼠,免疫60 d后,阴道接种鼠沙眼衣原体(Chlamydia muridarum,Cm)建立生殖道感染动物模型,在该模型中评价Pgp3蛋白疫苗抗Cm感染的保护效果,并探讨其机制。【结果】滴鼻或肌注免疫后,小鼠血清及生殖道中检测到了特异性抗体;小鼠脾淋巴细胞产生IFN-γ、IL-17及IL-5水平均明显高于对照组,且滴鼻免疫组IFN-γ水平升高较肌注组更显著(P<0.05);Pgp3蛋白滴鼻免疫组小鼠经Cm生殖道感染后,阴道带菌时间明显缩短,输卵管病理改变轻而肌注免疫组其保护作用不明显。【结论】Pgp3蛋白经滴鼻免疫可有效诱导小鼠产生明显的抗Cm保护效应。其可能的免疫保护机制与诱导Th1型为主的细胞免疫应答及高效价的特异性抗体有关,提示Pgp3蛋白疫苗具有潜在的疫苗研究与开发价值。
【Objective】 To investigate the immunoprotective effect of Pgp3 secreted by different immunization routes of Chlamydia trachomatis (Ct) and to analyze its possible protective mechanism to determine the value of Pgp3 protein vaccine in the development of Ct vaccine. 【Method】 Female Balb / c mice were immunized intranasally or intramuscularly with secretory protein Pgp3. Chlamydia muridarum (Cm) was intravaginally inoculated 60 days after immunization to establish animal model of genital tract infection. The model was evaluated in this model Protective effect of Pgp3 protein vaccine against Cm infection and its mechanism. 【Results】 Specific antibodies were detected in the serum and genital tract of mice after intranasal or intramuscular immunization. The levels of IFN-γ, IL-17 and IL-5 produced by splenic lymphocytes in mice were significantly higher than those in control group The level of IFN-γ in nasal immunization group was significantly higher than that in intramuscular injection group (P <0.05). The vaginal delivery time of mice in Pgp3 immunized group was significantly shorter than that in intramuscular injection group Group its protective effect is not obvious. 【Conclusion】 Pgp3 protein can effectively induce mouse anti-Cm protective effect through intranasal immunization. The possible mechanism of immune protection is related to the induction of Th1-based cellular immune response and high titer specific antibody, suggesting that Pgp3 protein vaccine has potential vaccine research and developmental value.