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目的:建立参术康胃胶囊的质量标准。方法:采用薄层色谱法对参术康胃胶囊中黄连、苦参、大青叶、延胡索进行定性鉴别;采用高效液相色谱法测定参术康胃胶囊中盐酸小檗碱的含量。结果:苦参、黄连、大青叶、延胡索的薄层色谱斑点清晰、分离效果较好、且无阴性干扰。盐酸小檗碱在0.089 1~0.712 4μg范围内与其峰面积积分值呈良好线性关系(r=0.999 9),平均加样回收率为98.27%,RSD=1.89%(n=6)。结论:该方法简便可行、专属性强、重复性好,可用于参术康胃胶囊的质量控制。
Objective: To establish the quality standard of Shenzhikangwei capsule. Methods: The TLC, HPLC, HPLC, HPLC method was used to identify the content of berberine hydrochloride in Shenzhikangwei Capsules. Results: Sophora flavescens, Coptis chinensis, Folium, Corydalis thin TLC spots clear separation effect is good, and no negative interference. Berberine hydrochloride had a good linear relationship with the peak area integral value (r = 0.999 9) in the range of 0.089 1 to 0.712 4 μg with an average recovery of 98.27% and RSD = 1.89% (n = 6). Conclusion: This method is simple, feasible, specific and reproducible. It can be used for quality control of Shenschikang Capsules.