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目的观察镉染毒动物模型接种肿瘤或给予激素后尿镉水平的改变,探讨肿瘤及激素是否影响机体排镉。方法选取无特定病原体级KM小鼠40只,按体质量随机分为阴性对照组、染镉组、染镉+接种肿瘤组和染镉+雌激素组,每组10只,雌雄各半。染镉组、染镉+接种肿瘤组、染镉+雌激素组均给予腹腔注射氯化镉+巯基乙醇混合液,连续5 d,第6天检测尿镉和尿肌酐,第16天后2组分别一次性接种S180肿瘤细胞株或腹腔注射雌激素,第26天重复检测尿镉和尿肌酐,并取各组小鼠肝、肾、脑等脏器分别进行病理检查。结果第6天和第26天的统计结果与阴性对照组相比,各处理组的尿肌酐差异均无统计学意义(P>0.05);染镉+接种肿瘤组尿镉排泄明显增快,但与染镉组比较,尿镉排泄差异无统计学意义(P>0.05),该组有明显的肾小管出血及坏死。结论本实验结果显示,急性镉染毒可导致肾脏损伤,肿瘤或内分泌激素对机体排镉作用未见明显的影响。
Objective To observe the changes of urinary cadmium levels after inoculation of tumors or hormones in cadmium-exposed animal models and to explore whether tumors and hormones affect the body’s ability to discharge cadmium. Methods Forty KM mice without specific pathogen were selected and randomly divided into negative control group, cadmium group, cadmium + tumor inoculation group and cadmium + estrogen group with 10 mice in each group, male and female were divided into two groups. Cadmium, cadmium + tumor inoculation group, cadmium + estrogen group were given intraperitoneal injection of cadmium chloride + mercaptoethanol mixed solution for 5 days, on the 6th day urinary cadmium and urinary creatinine, the first 16 days after the two groups were S180 tumor cells were inoculated once or intraperitoneally with estrogen. On the 26th day, urinary cadmium and urinary creatinine were detected repeatedly. Liver, kidney, brain and other organs in each group were examined by pathology. Results On the 6th day and the 26th day, the urinary creatinine had no statistical difference between the treatment groups and the negative control group (P> 0.05). Excretion of urinary cadmium in cadmium + Compared with the cadmium-exposed group, urinary cadmium excretion had no significant difference (P> 0.05). The group had obvious tubular hemorrhage and necrosis. Conclusion The experimental results show that acute cadmium exposure can cause kidney damage, tumor or endocrine hormone on the body of cadmium discharge had no significant effect.