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目的建立同时测定玉叶金花清热片中栀子苷、穿心莲内酯和脱水穿心莲内酯含量的HPLC。方法采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.2%磷酸水溶液,梯度洗脱,流速为1.0 m L·min?1,检测波长:238 nm(0~10 min,栀子苷),225 nm(10~22 min,穿心莲内酯),254 nm(22~35 min)。结果栀子苷在0.166~2.981μg内线性关系良好(r=0.999 9),平均回收率为99.4%(RSD=1.2%);穿心莲内酯在0.146~2.621μg内线性关系良好(r=0.999 9,平均回收率为99.2%(RSD=1.3%);脱水穿心莲内酯在0.222~3.992μg内性关系良好(r=0.999 7),平均回收率为100.1%(RSD=1.4%)。结论该方法简便、快捷、准确,可用于该制剂的质量控制。
Objective To establish a HPLC method for simultaneous determination of geniposide, andrographolide and dehydroandrographolide in jade leaves Jinhua Qingre tablets. Methods The mobile phase was methanol-0.2% phosphoric acid aqueous solution with gradient elution at a flow rate of 1.0 m L · min -1 on an Agilent ZORBAX SB-C18 column (4.6 mm × 150 mm, 5 μm). The detection wavelength was 238 nm ~ 10 min, geniposide), 225 nm (10-22 min, andrographolide), 254 nm (22-35 min). Results Geniposide had good linearity (r = 0.999 9) within 0.166-2.981 μg with an average recovery of 99.4% (RSD = 1.2%). Andrographolide showed a good linearity within 0.146-2.621 μg (r = 0.999 9 , With an average recovery of 99.2% (RSD = 1.3%). The average recovery of dehydroandrographolide was 0.222 ~ 3.992μg (r = 0.999 7) with an average recovery of 100.1% (RSD = 1.4% Simple, fast, accurate, can be used for the quality control of the preparation.