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目的:研究不同疾病进展阶段的中国HIV/AIDS患者外周血CD8+T细胞亚群的数量变化,以探讨各亚群与HIV感染疾病进展的相关性。方法:首先应用多参数(CD45RA、CCR7、CD28)流式细胞术圈定幼稚(nave)CD8+T细胞和记忆性(memory)CD8+T细胞(包括TCM、TEM-1及TEM-2),随后测定58例不同疾病进展阶段HIV/AIDS患者和20例健康对照者外周血各CD8+T细胞亚群的绝对数量和占CD8+T细胞的百分比,分析其与病毒载量、CD4+T细胞数量的相关性。结果:HIV/AIDS患者与健康对照相比,nave CD8+T细胞百分比和绝对值均显著减少,memory CD8+T细胞百分比和绝对值均显著升高(P<0.05)。在memoryCD8+T细胞各亚群中,效应型(effector memory T cells,TEM)-1百分比显著升高(P<0.05)。HIV感染缓慢进展(slow progressor,SP)组、HIV组及AIDS组中,nave CD8+T细胞百分比和绝对值均依次下降,AIDS组、HIV组显著低于SP组(P<0.05),memory CD8+T细胞绝对值依次下降,各组间的下降均有显著性差异(P<0.05)。在memory CD8+T细胞各亚群中,TEM-1百分比依次升高,AIDS组显著高于SP组(P<0.05);TEM-2百分比及绝对值依次下降,AIDS组显著低于SP组(P<0.05)。相关性分析发现,nave CD8+T细胞与CD4+T细胞显著正相关(P<0.01),与HIV病毒载量显著负相关(P<0.01);中央型(central memory T cells,TCM)和TEM-2型memory CD8+T细胞与CD4+T细胞显著正相关(P<0.01)。结论:Nave、memory CD8+T细胞数量与HIV疾病进展显著相关。
Objective: To study the changes of the number of CD8 + T cell subsets in peripheral blood of HIV / AIDS patients in different stages of disease progression in order to explore the correlation between each subpopulation and the progression of HIV infection. Methods: Nave CD8 + T cells and memory CD8 + T cells (including TCM, TEM-1 and TEM-2) were initially delineated by multi-parameter flow cytometry (CD45RA, CCR7, CD28) The absolute number of CD8 + T cell subsets and the percentage of CD8 + T cells in peripheral blood of 58 AIDS patients with HIV / AIDS and 20 healthy controls were determined. The relationship between viral load, CD4 + T cell count Relevance. Results: Compared with healthy controls, the percentage and absolute value of nave CD8 + T cells in HIV / AIDS patients were significantly decreased, and the percentage and absolute value of memory CD8 + T cells were significantly increased (P <0.05). In each subset of memoryCD8 + T cells, the percentage of effector memory T cells (TEM) -1 was significantly increased (P <0.05). The percentages and absolute values of nave CD8 + T cells in HIV / AIDS group and AIDS group were successively decreased in AIDS progression group (HIV), AIDS group and HIV group (P <0.05), and memory CD8 + T cells in descending order, the decline between the groups were significantly different (P <0.05). In the memory CD8 + T cell subsets, the percentage of TEM-1 increased in turn, the AIDS group was significantly higher than the SP group (P <0.05); the percentage of TEM-2 and the absolute value decreased in turn, AIDS group was significantly lower than the SP group P <0.05). Correlation analysis showed that there was a significant positive correlation between nave CD8 + T cells and CD4 + T cells (P <0.01), negatively correlated with HIV viral load (P <0.01), central memory T cells (TCM) and TEM -2 memory CD8 + T cells were significantly and positively correlated with CD4 + T cells (P <0.01). Conclusion: The number of CD8 + T cells in Nave and memory is significantly correlated with the progression of HIV disease.