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用地高辛标记HBV-DNA探针原位杂交技术检测了25例肝细胞性肝癌组织内HBV-DNA的分布状态。结果表明,25例中原位杂交阳性者20例(80%),有HBV血清学标志的23例中,19例癌组织原位杂交阳性(82.6%)。无HBV血清学标志的2例中,癌组织有1例原位杂交阳性。HBV-DNA在癌细胞内的分布以核内为主,也可见于胞浆中,因而可能有整合型和复制型两种形式。提示HBV感染与肝癌的发生发展密切相关,慢性HBV感染可能成为致癌或促癌的重要因素。
Digoxin-labeled HBV-DNA probe in situ hybridization was used to detect the distribution of HBV-DNA in 25 cases of hepatocellular carcinoma. The results showed that among the 25 cases with positive in situ hybridization in 20 cases (80%), of the 23 cases with HBV serological markers, 19 cases had positive in situ hybridization (82.6%). Of the 2 cases without HBV serological markers, 1 was positive for in situ hybridization in cancer tissues. The distribution of HBV-DNA in cancer cells is mainly in the nucleus and also in the cytoplasm, so there may be two forms of integration and replication. It is suggested that HBV infection is closely related to the occurrence and development of liver cancer. Chronic HBV infection may be an important factor in carcinogenesis or cancer promotion.