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目的探讨还原型谷胱甘肽干预高同型半胱氨酸血症致兔动脉粥样硬化的机制。方法将44只新西兰白兔随机分为4组:对照组以标准饲料100 g.d-1喂养,模型组以1%蛋氨酸饲料100 g.d-1喂养,低剂量药物组以1%蛋氨酸饲料100g.d-1喂养+还原型谷胱甘肽15mg.d-1肌注,高剂量药物组以1%蛋氨酸饲料100g.d-1喂养+还原型谷胱甘肽30mg.d-1肌注。于实验前和实验8周后耳中央动脉采血检测Hcy、TC、TG、CRP、SOD并对结果进行分析。结果模型组Hcy水平高于对照组,差异有统计学意义(P<0.05);模型组SOD活性降低,与对照组比较差异有统计学意义(P<0.05);模型组CRP值高于对照组,差异有统计学意义(P<0.05);经还原型谷胱甘肽治疗,模型组IMT高于对照组(P<0.05);低剂量药物干预组IMT较对照组有所增厚(P<0.05);高、低剂量药物干预组IMT与模型组比较,差异有统计学意义(P<0.05)。结论还原型谷胱甘肽通过抑制氧化应激、炎症反应阻止动脉粥样硬化的发生、发展。
Objective To investigate the effect of reduced glutathione on atherosclerosis in rabbits with hyperhomocysteinemia. Methods Forty-four New Zealand white rabbits were randomly divided into four groups: the control group was fed with 100 gd-1 standard diet, the model group was fed with 100 gd-1 methionine diet, the low-dose group was fed with 100 g.d- 1 fed + reduced glutathione 15mg.d-1 intramuscular injection, high-dose drug group feeding 1g methionine diet 100g.d-1 + glutathione 30mg.d-1 intramuscular injection. The levels of Hcy, TC, TG, CRP and SOD in the central aorta were measured before and 8 weeks after the experiment. The results were analyzed. Results The level of Hcy in the model group was higher than that in the control group (P <0.05). The SOD activity in the model group was lower than that in the control group (P <0.05), and the CRP in the model group was higher than that in the control group (P <0.05). After treatment with reduced glutathione, the IMT in the model group was higher than that in the control group (P <0.05). The IMT in the low dose group was thicker than that in the control group (P < 0.05). There was significant difference between IMT group and model group in high and low dose intervention group (P <0.05). Conclusion Reduced glutathione inhibits the occurrence and development of atherosclerosis by inhibiting oxidative stress and inflammation.