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目的研究Carbogen长时间吸入的安全性,对肿瘤乏氧的改善以及对32P肿瘤间质内放疗疗效的影响。方法①荷S180肿瘤小鼠10只,行吸入Carbogen前、后的99mTcHL91SPECT乏氧显像,比较两者TNT的变化。②正常小鼠20只,分为对照组和Carbogen吸入组,后者每日吸Carbogen2h,共24d。观察小鼠的日常活动、体重变化,及重要脏器病理学变化。③荷S180肿瘤小鼠40只,分为未治疗空气组、未治疗Carbogen组、32P治疗空气组、32P治疗Carbogen组,观察、对比各组小鼠肿瘤生长情况,检测抑瘤率和抑瘤曲线。结果①Carbogen吸入前后肿瘤99mTcHL91的TNT分别为1.872±0.391和1.354±0.189,差异有统计学意义(t=4.476,P<0.01);②未治疗空气组、未治疗Carbogen组小鼠间肿瘤生长速率无明显区别;32P治疗空气组、32P治疗Carbogen组小鼠在治疗后第12天抑瘤率分别为66.01%和69.43%,两者间无明显区别,在治疗后第24天,肿瘤体积分别为(2.728±0.469)和(2.237±0.603)cm3(t=2.128,P<0.05),肿瘤质量分别为(2.437±0.447)和(1.965±0.538)g(t=2.134,P<0.05)。③正常小鼠Carbogen长期吸入后日常活动、体重变化等与正常对照组间无明显区别,心、脑、肺、肝、脾、肾、小肠等脏器未见明显异常病理学改变。结论Carbogen可以明显改善肿瘤乏氧状况,中长期吸入对小鼠毒副作用较小,在一定程度上可以增加32P肿瘤间质内放疗的疗效。
Objective To study the long-term safety of Carbogen inhalation, the improvement of tumor hypoxia and the effect of radiotherapy on 32P tumor interstitial. Methods ① 10 mice bearing S180 tumors were subjected to 99mTcHL91SPECT hypoxic imaging before and after Carbogen inhalation. The TNT changes of the two groups were compared. ② Twenty normal mice were divided into control group and Carbogen inhalation group, and the latter inhaled Carbogen2h daily for 24 days. Observe the mice daily activities, body weight changes, and pathological changes of important organs. (3) 40 mice bearing S180 tumor were divided into untreated air group, untreated Carbogen group, 32P treated air group and 32P treated Carbogen group. The growth of mice in each group were observed and compared. The inhibition rate and tumor inhibition curve . Results ① The TNT of 99mTcHL91 before and after inhalation of Carbogen were 1.872 ± 0.391 and 1.354 ± 0.189, respectively, with significant difference (t = 4.476, P <0.01). ② The tumor growth rate of untreated air group and untreated Carbogen group was Obvious difference between the two groups; 32P treatment air group, 32P treatment Carbogen group on the 12th day after treatment, the tumor inhibition rates were 66.01% and 69.43%, no significant difference between the two groups at 24 days after treatment, tumor volume were ( 2.728 ± 0.469) and (2.237 ± 0.603) cm3 respectively (t = 2.128, P <0.05). The tumor mass was (2.437 ± 0.447) and (1.965 ± 0.538) g (t = 2.134, P <0.05) respectively. ③ There was no significant difference between the normal mice and the control group after long-term inhalation of Carbogen. There were no obvious abnormal pathological changes in heart, brain, lung, liver, spleen, kidney and small intestine. Conclusion Carbogen can significantly improve the hypoxia status of the tumor. Long-term inhalation of Carbogen has less toxic side effects on mice and can increase the therapeutic effect of 32P tumor interstitial radiotherapy to a certain extent.