论文部分内容阅读
目的探讨肠内肠外营养与经肠道补充益生菌对腹腔感染大鼠肠道微生态及肠屏障功能的影响。方法21只SD大鼠随机分为3组(每组7只),分别给予肠外营养(PN组)、肠外加肠内营养(PN加EN组)和肠外、肠内营养加益生菌(益生菌组)。3组营养供给为等热、等氮量。于第6天处死大鼠,取其盲肠内容物作厌氧菌培养,采用随机扩增多态性DNA技术作菌种DNA指纹图谱分析;采用免疫组织化学方法测定其末段回肠和结肠的跨膜结合蛋白及肠上皮浆细胞免疫球蛋白(IgA)表达水平;取腔静脉血及肺、肝、肠系膜淋巴组织匀浆后作细菌培养,测细菌易位率;采用鲎试剂法检测门静脉血内毒素含量。结果(1)益生菌组及PN加EN组各种菌种的数量均较PN组增多(P<0.05)。PN组细菌DNA指纹图谱条带明显减少,且出现明显异常条带,其他两组大鼠肠道内优势菌群的基因条带与正常大鼠具有较高的一致性。(2)PN加EN组和益生菌组小肠和结直肠跨膜结合蛋白及IgA表达明显高于PN组(P<0.05及P<0.01),且益生菌组跨膜结合蛋白的表达高于PN加EN组(P<0.05);PN加EN组的小肠和益生菌组结直肠IgA的表达明显高于PN组(P<0.01)。(3)PN加EN组和益生菌组血、肺、肝、肠系膜淋巴组织的细菌易位率和内毒素水平均低于PN组(P<0.05),前两组之间差异无统计学意义。结论益生菌联合EN能增加肠上皮跨膜结合蛋白IgA表达,改善肠道微生态,从而保护肠黏膜屏障、减少细菌易位。
Objective To investigate the effects of enteral parenteral nutrition and enteral supplement of probiotics on intestinal microflora and intestinal barrier function in rats with abdominal infection. Methods Twenty-one Sprague-Dawley rats were randomly divided into three groups (n = 7 each). The rats were given parenteral nutrition (PN), parenteral nutrition (PN plus EN) and parenteral, enteral nutrition plus probiotics Probiotic group). 3 groups of nutrients such as heat supply, such as nitrogen. The rats were sacrificed on the 6th day, the cecal contents were taken as anaerobic bacteria, and DNA fingerprinting was performed by using random amplified polymorphic DNA (PCR). The ileum and colonic cross-sections were determined by immunohistochemistry Membrane-bound protein and intestinal epithelial cell immunoglobulin (IgA) expression levels; take vena cava and lung, liver and mesentery lymphoid tissue homogenates for bacterial culture, bacterial translocation rate was measured; 鲎 reagent used to detect portal vein blood Toxin content. Results (1) Compared with PN group, the number of various strains in probiotics group and PN plus EN group increased (P <0.05). The bacterial DNA fingerprinting bands of PN group were significantly reduced and obvious abnormal bands were observed. The gene bands of dominant bacteria in the intestine of the other two groups were highly consistent with normal rats. (2) The expressions of small intestine and colorectal transmembrane-associated protein and IgA in PN plus EN group and probiotics group were significantly higher than those in PN group (P <0.05 and P <0.01), and the expression of transmembrane-associated protein in probiotic group was higher than that in PN group (P <0.05). The expression of IgA in colorectal and probiotic groups of PN + EN group was significantly higher than that in PN group (P <0.01). (3) The bacterial translocation rate and endotoxin level in the blood, lung, liver and mesentery lymphoid tissues in PN plus EN group and probiotic group were lower than those in PN group (P <0.05), and there was no significant difference between the two groups . Conclusion Probiotics combined with EN can increase the expression of transmembrane-associated protein IgA in gut epithelium and improve gut microflora, thus protecting intestinal mucosal barrier and reducing bacterial translocation.