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为了探讨柯萨奇 B 组病毒感染与云南慢型克山病的关系,本文用缺口平移法制备生物素标记的柯萨奇 B_3病毒的 cDNA 探针与云南慢型克山病尸检心肌组织15例及脑外伤等非正常死亡的尸检心肌组织10例石腊切片及引产胎儿心肌组织10例的石腊切片进行原位杂交。结果云南慢克16例中有11例出现阳性杂交信号,即有柯萨奇 B 组病毒(CVB)RNA 存在,阳性率达73.3%。脑外伤等非正常死亡的心肌及胎心均为阴性。提示云南慢型克山病与 CVB 的感染高度相关。
In order to investigate the relationship between coxsackie B virus infection and chronic Keshan disease in Yunnan, a cDNA probe of biotin-labeled coxsackie B_3 virus and autopsy myocardium of chronic Keshan disease in Yunnan And brain trauma and other abnormal death of autopsy myocardial tissue 10 cases of paraffin section and induction of fetal myocardial tissue 10 cases of paraffin sections in situ hybridization. Results The positive hybridization signal was found in 11 of 16 cases in Yunji of Yunnan Province. The positive rate of Coxsackie B virus (CVB) RNA was 73.3%. Brain trauma and other abnormal death of the heart and fetal heart rate were negative. Tip Yunnan slow Keshan disease and CVB infection are highly correlated.