论文部分内容阅读
目的采用HPLC法测定复方北豆根氨酚那敏片中咖啡因、马来酸氯苯那敏与对乙酰氨基酚的含量,并检测咖啡因与马来酸氯苯那敏的含量均匀度。方法采用C_(18)色谱柱(250 mm×4.6 mm,5μm),以p H4.0磷酸二氢钾溶液-甲醇为流动相,梯度洗脱,检测波长为225 nm(咖啡因与马来酸氯苯那敏)、254 nm(对乙酰氨基酚),流速1.0 m L·min~(-1),柱温30℃,进样量20μL。结果咖啡因、马来酸氯苯那敏和对乙酰氨基酚浓度的线性范围分别为72.41~217.2、14.81~44.44、31.06~93.18μg·m L~(-1),平均回收率分别为100.58%、99.18%、99.64%(n=9)。结论所用法简便、准确,可同时测定多种组分,可用于该制剂的质量控制。
OBJECTIVE To determine the contents of caffeine, chlorpheniramine maleate and acetaminophen in Compound Beisuan Root Paracetamol Tablets by HPLC and determine the content uniformity of caffeine and chlorpheniramine maleate. Methods C18 column (250 mm × 4.6 mm, 5 μm) was used as the mobile phase with p H4.0 potassium dihydrogen phosphate solution-methanol as the mobile phase. The detection wavelength was 225 nm (caffeine and maleic acid Chlorpheniramine), 254 nm (paracetamol), the flow rate was 1.0 m L · min -1, the column temperature was 30 ℃ and the injection volume was 20 μL. Results The linear ranges of caffeine, chlorpheniramine maleate and acetaminophen were 72.41 ~ 217.2, 14.81 ~ 44.44 and 31.06 ~ 93.18 μg · m L -1, respectively, and the average recoveries were 100.58% 99.18%, 99.64% (n = 9). Conclusion The method used is simple, accurate and can be used for simultaneous determination of various components for the quality control of this preparation.