目的观察Nogo-A在少突胶质前体细胞的表达和细胞定位。方法采用改良振荡分离纯化法及化学限定培养基体外培养少突胶质前体细胞,并用其特异性抗体A2B5、O4、O1用免疫荧光法作细胞鉴定。然后在相应的少突胶质细胞分化阶段用免疫荧光法观察Nogo-A的表达。结果Nogo-A在细胞分离纯化培养后的1、2、4d均有阳性表达,阳性信号位于胞体及突起。结论在少突胶质祖细胞及未成熟少突胶质细胞表面及突起上均表达Nogo-A,为早产儿脑白质损伤及神经系统后遗症的发生机制中的作用提供了实验室基础。 Objective To observe the expression and cell localization of Nogo-A in oligodendrocyte precursor cells. Methods Oligodendrocyte precursor cells were cultured in vitro by modified oscillatory separation and purification and chemically defined medium. The specific antibodies A2B5, O4 and O1 were identified by immunofluorescence. The expression of Nogo-A was then observed by immunofluorescence at the corresponding oligodendrocyte differentiation stage. Results Nogo-A was expressed on the 1st, 2nd, and 4th day after isolation and purification of cells. The positive signals were located in the cell bodies and protrusions. Conclusion The expression of Nogo-A on the surface and prominence of oligodendrocyte progenitor cells and immature oligodendrocytes provided a laboratory basis for the role of the pathogenesis of white matter damage and neurological sequelae in preterm infants.