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Objective To investigate the effects of TGF- β1 on proliferation and type N collagen mRNAexpression of rat renal interstitial libroblasts in vitro for elucidating the role of fibroblasts in renal interstitialfibrosis. Methods The cells were cultured in media containing various concentrations of TGF- β1. Theproliferation and the type Ⅲ collagen mRNA expression of the cells were assayed with MTT method andRT- PCR respectively. Results TGF- β1, has a stimulating proliferation effect with dose- dependence and aincreasing expression of type Ⅲ collagen mRNA with both dose- and time- dependence to the renal fibroblasts. Conclusion It is suggested that TGF- β, is involved in proliferation of renal fibroblasts and their type Ⅲcollagen mRNA expression, and may play an important role in renal interstitial fibrosis.
Objective To investigate the effects of TGF-β1 on proliferation and type N collagen mRNA expression of rat renal interstitial libroblasts in vitro for elucidating the role of fibroblasts in renal interstitial fibrosis. Methods The cells were cultured in media containing various concentrations of TGF- β1. The proliferation and the type III collagen mRNA expression of the cells were assayed with MTT method and RT-PCR respectively. Results TGF-β1, has a stimulating proliferation effect with dose- dependence and a proteincreting expression of type III collagen mRNA with both dose- and time- dependence to The renal fibroblasts. Conclusion It is suggested that TGF- [beta], is involved in proliferation of renal fibroblasts and their type III colagen mRNA expression, and may play an important role in renal interstitial fibrosis.