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目的了解东莞市不同来源的副溶血性弧菌的病原学特征,为疾病防控提供实验依据。方法实验菌株来源为2012—2013年分别从东莞市食品监测、食物中毒患者及散发病例中分离的43株副溶血性弧菌。根据GB 4789.7—2013对43株副溶血菌株进行生化鉴定及血清学分析;运用K-B纸片法对菌株进行12种抗生素药敏试验;应用实时荧光PCR扩增法检测菌株的tdh、trh基因;并经限制性内切酶NotⅠ酶切后进行脉冲场凝胶电泳(PFGE)分子分型。结果 43株副溶血菌株中,食物中毒患者、散发病例分离株血清型主要为O3∶K6,占所有病例分离株的83.3%(15/18),25株食品分离株血清型呈多样化分布,无优势血清型;43株副溶血性弧菌对氨苄西林(敏感率为0.0%,0/43)和头孢噻吩(敏感率为20.9%,9/43)敏感率低,对环丙沙星、磺胺复合、亚胺培南完全敏感(敏感率为100.0%,43/43);毒力基因结果显示,病例分离株均只携带tdh基因,食品分离株均不携带tdh、trh基因;PFGE分子分型结果显示,腹泻散发病例、食物中毒患者分离株带型相对集中,食品分离株带型相对分散,相同血清型的菌株同源性较高,不同食物中毒患者分离株PFGE带型一致。结论食品分离株种类繁多且致病性较低,病例分离株同源性较高且致病性较高。
Objective To understand the etiological characteristics of Vibrio parahaemolyticus from different sources in Dongguan and provide experimental evidence for disease prevention and control. Methods The source of the experimental strains was 43 strains of Vibrio parahaemolyticus isolated from food surveillance, food poisoning patients and sporadic cases in Dongguan from 2012 to 2013 respectively. According to GB 4789.7-2013, 43 strains of parahaemolytic strains were biochemically identified and serologically analyzed; 12 antibiotic susceptibility tests of strains were carried out by KB paper method; the tdh and trh genes of strains were detected by real-time fluorescence PCR amplification; After digestion with restriction endonuclease Not I, PFGE molecular typing was performed. Results Among the 43 strains of isolates, the serotypes of patients with food poisoning and sporadic cases were mainly O3: K6, accounting for 83.3% (15/18) of all case isolates. The serotypes of 25 isolates were diversified, 43 strains of Vibrio parahaemolyticus were sensitive to ampicillin (0%, 0/43) and cefalotin (20.9%, 9/43) Sulfonamides, and imipenem (sensitivity rates 100.0%, 43/43). The results of virulence genes showed that all the isolates contained tdh gene and none of the isolates contained tdh and trh genes. PFGE The results showed that cases of diarrhea sporadic cases, food poisoning patients with relatively concentrated band type, food isolates with relatively dispersed, strains of the same serotype homology higher, isolates of different food poisoning PFGE band type consistent. Conclusions A wide range of food isolates have low pathogenicity, and the isolates have high homology and high pathogenicity.