论文部分内容阅读
为了解CIII-1抗菌蛋白对辣椒炭疽病的防病机理,研究了用CIII-1抗菌蛋白处理后的辣椒果内可溶性蛋白、丙二醛(MDA)含量及过氧化物酶(POD)、超氧化物歧化酶(SOD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性变化。结果表明:CIII-1抗菌蛋白粗提液喷雾后立即接种辣椒炭疽,9天的防治效果为81.82%,13天仍有52.00%;抗菌蛋白处理辣椒果后,可溶性蛋白及MDA含量和超氧化物歧化酶(SOD)变化不明显,其它3种防御酶在辣椒体内都有明显提高,但各酶活提高程度、酶活高峰出现的时间不同,说明不同的酶在辣椒体内发挥作用的时间不同。因此,CIII-1抗菌蛋白可通过诱导植株防御酶系活性的提高增强植株的抗病性。
In order to understand the mechanism of CIII-1 anti-bacterial protein on pepper anthracnose, we studied the content of soluble protein, malondialdehyde (MDA) and peroxidase (POD) in capsicum treated with CIII- The activities of SOD, PPO and PAL were measured. The results showed that the anthrax was inoculated immediately after spraying the CIII-1 antibacterial protein crude extract, the control efficacy was 81.82% at 9 days and 52.00% at 13 days. The content of soluble protein, MDA and superoxide The changes of SOD and SOD were not obvious. The other three kinds of defense enzymes increased significantly in pepper body. However, the extent of each enzyme activity increased and the time of peak appeared different, indicating that different enzymes played different roles in pepper body. Therefore, the CIII-1 antimicrobial protein can enhance the plant’s disease resistance by inducing the increase of plant defense enzyme activity.