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目的:研究不同剂量培哚普利对兔急性心肌梗死(AMI)后循环血中内皮祖细胞(EPCs)数量的影响,及循环血血浆中血管内皮生长因子(VEGF)、血管紧张素Ⅱ(AngⅡ)及血管紧张素1~7(Ang1~7)含量的变化。方法:选择清洁级(2.2±0.3)kg新西兰大白兔,开胸及部分结扎左前降支处理,将存活的实验用兔随机分为四组,A组为假手术组(n=7);B组为AMI组(n=6);C组为小剂量培哚普利组(n=6);D组为大剂量培哚普利组(n=6)。分别于1、3、7、14、28天采血,用流式细胞仪计数循环血中内皮祖细胞(EPCs)的数量,酶联免疫吸附法(ELISA)检测循环血中VEGF、AngⅡ、Ang1~7的含量。结果:AMI后,四组外周循环血中EPCs数量、VEGF的含量随着时间的推移均逐渐增加,于第7天达到高峰,之后逐渐降低,D组上述趋势变化最明显,且28天时D组较B组、C组仍能维持在较高水平[EPCs:(0.066±0.005)% vs (0.059±0.005)%,(0.062±0.003)%;VEGF(114.692±9.599)pg/ml vs (84.058±4.608)pg/ml,(102.570±10.869)pg/ml],差异均有统计学意义(P均<0.05);四组AngⅡ的含量增多,于第3天达到高峰,之后逐渐下降,下降幅度以D组最为明显;Ang1~7在手术组第1天有明显下降趋势,之后逐渐增多,随着时间推移呈逐渐增多的趋势,以D组更为明显。结论 :培哚普利可促进AMI兔外周循环血中EPCs的动员及VEGF、Ang1~7含量的增多,降低AngⅡ的水平,且应用大剂量培哚普利干预效果更明显。
OBJECTIVE: To study the effects of different doses of perindopril on the number of endothelial progenitor cells (EPCs) in circulating blood after acute myocardial infarction (AMI) in rabbits, and the levels of vascular endothelial growth factor (VEGF), angiotensin Ⅱ ) And angiotensin 1 ~ 7 (Ang1 ~ 7) content changes. Methods: New Zealand white rabbits (2.2 ± 0.3) kg were selected and thoracotomy and partial ligation of the left anterior descending branch were performed. Survival rabbits were randomly divided into four groups, group A was sham operation group (n = 7), B Groups were AMI (n = 6); group C was low-dose perindopril (n = 6); group D was high-dose perindopril (n = 6). The blood samples were collected on days 1, 3, 7, 14, and 28. The numbers of circulating endothelial progenitor cells (EPCs) were counted by flow cytometry. The levels of VEGF, AngⅡ, 7 content. Results: After AMI, the number of EPCs and the content of VEGF in all the four groups increased gradually with the passage of time, reached its peak on the 7th day and then gradually decreased. The above trend of D group was the most obvious, and on the 28th day, Compared with group B, group C still maintained at a relatively high level [EPCs: (0.066 ± 0.005)% vs (0.059 ± 0.005)%, (0.062 ± 0.003)% vs VEGF (114.692 ± 9.599) pg / ml vs (84.058 ± 4.608) pg / ml, (102.570 ± 10.869) pg / ml], the differences were statistically significant (all P <0.05). The content of AngⅡin the four groups increased and peaked on the third day, then decreased gradually D group was the most obvious; Ang1 ~ 7 in the operation group on the first day there was a significant downward trend, then gradually increased, with the gradual increase in the trend over time to D group is more obvious. CONCLUSION: Perindopril can promote the mobilization of EPCs and the increase of VEGF, Ang1 ~ 7 levels in peripheral blood of AMI rabbits and decrease the level of AngⅡ. The effect of perindopril at high dose is more obvious.