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通过新疆喀什地区3种典型植被环境(红柳区、灌木区、胡杨区)采集的土样分析,研究不同植被覆盖条件下土壤微生物的分布情况及影响该地区土壤微生物分布状况的主要因素,并分离、筛选出其中的优势菌株。结果表明,微生物的总数为红柳区>灌木区>胡杨区;细菌数量远大于放线菌及霉菌的数量;红柳区细菌数量最多,占3个采样点细菌总数的69.35%,其次为灌木区、胡杨区;3个采样点的放线菌数量相近;红柳区及灌木区霉菌数量最多,均为胡杨区霉菌数量的2倍;同种植被条件下,细菌、放线菌、真菌数量随着土壤深度增大变化趋势无明显规律;植物根部表面土壤中微生物数量是其周围土壤中微生物数量的1.5～11.0倍。分离出的优势菌株经Biolog自动鉴定系统及16S rDNA鉴定结果:XJ-H、XJ-DH、XJ-ZH为萎缩芽孢杆菌(Bacillus atrophaeus),XJ-QT和XJ-R为枯草杆菌(Bacillus subtilis),XJ-BZ为斯氏假单胞菌(Pseudomona stutzeri)。
Through the analysis of soil samples collected from three typical vegetation environments (Hongliu district, shrub area and Huyang district) in Kashgar region of Xinjiang, the distribution of soil microorganisms under different vegetation cover conditions and the main factors affecting the distribution of soil microorganisms in the area were studied and separated , Which selected the dominant strains. The results showed that the total number of microorganisms was in Hongliu area> shrub area> Populus euphratica area. The number of bacteria was much larger than that of actinomycetes and molds. The quantity of bacteria in Hongliu area was the highest, accounting for 69.35% of the total number of bacteria in three sampling sites, followed by shrub area, Populus euphratica and Populus euphratica. The number of actinomycetes in three sampling sites was similar. The number of fungi in Hongliu district and shrub area were the most, which were two times that of Populus euphratica. Under the same vegetation condition, the number of bacteria, actinomycetes and fungi varied with the soil There was no obvious regularity of the trend of increasing depth. The number of microorganisms in the soil surface of plant roots was 1.5 ~ 11.0 times of that of the soil in the surrounding soil. The dominant strains isolated by Biolog identification system and 16S rDNA identification results: XJ-H, XJ-DH, XJ-ZH Bacillus atrophaeus (Bacillus atrophaeus), XJ-QT and XJ-R Bacillus subtilis (Bacillus subtilis) , XJ-BZ is Pseudomonas stutzeri.