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含发光酶基因luxAB的Tn5转座子自杀质粒pHNC3，在辅助质粒pRK2013的帮助下，转入快生型大豆根瘤菌HN01中小，pHNC3经自杀重组，其Tn5－luxAB转座插入HN01基因组中，从而赋予HN01以发光活性。挑取具有发光活性的HN01杂交单菌落，进行质粒快检和以luxAB为探针的分子杂交，选取Tn5-luxAB分别插入到HN01染色体上和不同质粒上的标记菌株，进行灭菌盆栽实验，并对一株Tn5-luxAB标记于染色体上的菌株HN01LC02进行了模拟大豆栽培条件下的有菌盆栽实验，包括对发光根瘤菌占瘤率的测定和发光根瘤在根系上分布情况的测定。
Tn5 transposon containing luxAB gene suicide plasmid pHNC3 was transferred into fast-growing soybean soybean HN01 with the help of helper plasmid pRK2013, and pHNC3 was inserted into HN01 genome by suicide recombination. HN01 is endowed with luminescent activity. Single colonies of HN01 hybrids with luminescent activity were picked and subjected to rapid plasmid detection and luxAB probe hybridization. Tn5-luxAB was inserted into the HN01 chromosome and tagged strains on different plasmids respectively for sterilized pot experiments A strain of HN01LC02 with Tn5-luxAB on chromosome was used to simulate the cultivation of bacteria in soybean plants, which included the determination of the rate of luminescent Rhizobia and the distribution of luminescent nodules on the root system.