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目的:建立HPLC法测定肝血瘤康糖浆中芍药苷含量的方法。方法:采用DIONEX C18色谱柱(250 mm×4.6 mm,5μm),甲醇-0.05mol.L-1磷酸二氢钾溶液(38:62)为流动相,流速:1.0ml.min-1,柱温:30℃,检测波长:230 nm。结果:芍药苷在0.12～5.99μg(r=0.999 8)范围内呈良好的线性关系,平均回收率为100.4%(RSD=0.8%,n=6)。结论:本方法灵敏、简便、准确,可用于肝血瘤康糖浆中芍药苷的含量控制。
Objective: To establish a HPLC method for the determination of paeoniflorin in Hematuria and Syrup. Methods: The mobile phase consisted of DIONEX C18 column (250 mm × 4.6 mm, 5 μm), methanol-0.05 mol·L-1 potassium dihydrogen phosphate solution (38:62), flow rate of 1.0 ml · min-1, : 30 ℃, detection wavelength: 230 nm. Results: Paeoniflorin had a good linear relationship in the range of 0.12 ~ 5.99 μg (r = 0.999 8) with an average recovery of 100.4% (RSD = 0.8%, n = 6). Conclusion: The method is sensitive, simple and accurate, and can be used for the content control of paeoniflorin in Hematuria and syrup.