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目的研究大鼠局灶性脑缺血－再灌注期细胞间粘附分子－１（ＩＣＡＭ－１）在转录水平表达规律。方法用插线法建立大鼠局灶性脑缺血再灌注模型，用逆转录－聚合酶链式反应（ＲＴ－ＰＣＲ），测定ＩＣＡＭ－１转录水平（ＩＣＡＭ－１ｍＲＮＡ）的表达。结果发现ＩＣＡＭ－１ｍＲＮＡ在缺血２ｈ升高，至缺血再灌注１０ｈ升至高峰，持续１周。结论ＩＣＡＭ－１在脑缺血再灌注时的表达明显上调，介导了白细胞、脑微血管内皮细胞的粘附增强，和脑缺血再灌注损伤关系密切。
Objective To investigate the expression of intercellular adhesion molecule-1 (ICAM-1) at focal cerebral ischemia-reperfusion in rats. Methods Focal cerebral ischemia-reperfusion model was established by the method of patch-line. The expression of ICAM-1 mRNA was determined by reverse transcription-polymerase chain reaction (RT-PCR) ICAM-1mRNA was found to be elevated at 2h after ischemia and peaked at 10h after ischemia-reperfusion for 1 week. Conclusion The expression of ICAM-1 is significantly up-regulated during cerebral ischemia-reperfusion, which leads to the enhanced adhesion of leukocytes and cerebral microvascular endothelial cells, which is closely related to cerebral ischemia-reperfusion injury.