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研究125I粒子诱导体外培养的人食管癌Eca-109细胞凋亡及对其细胞周期的影响。设置A组:对照(不加粒子),B组:低剂量(7.4×106Bq125I粒子1枚),C组:中剂量(14.8×106Bq125I粒子1枚),D组:高剂量(29.6×106Bq125I粒子1枚),细胞培养1周后收集细胞,流式细胞术检测细胞凋亡及细胞周期。4组凋亡细胞阳性指数(AI)分别为0.17%、1.17%、4.35%、4.72%,B、C和D组与A组之间均有非常显著性差异(P<0.01);C组与B组,D组与B组比较,均有非常显著性差异(P<0.01),D组与C组比较无显著性差异(P>0.05)。随粒子剂量增加,G2/M期细胞所占比例增高,各组之间有非常显著性差异(P<0.01)。125I粒子可以诱导体外培养的人食管癌Eca-109细胞凋亡,其诱导凋亡的能力可能是通过把细胞阻滞在G2/M期实现。
To study the apoptosis of human esophageal cancer Eca-109 cells induced by 125I particles and its effect on cell cycle. Group B: low dose (7.4 × 106 Bq125I particles), Group C: medium dose (14.8 × 106 Bq125I particles), Group D: high dose (29.6 × 106 Bq125I particles 1 ), Cells were collected after 1 week culture, and apoptosis and cell cycle were detected by flow cytometry. The positive index (AI) of apoptotic cells in 4 groups were 0.17%, 1.17%, 4.35% and 4.72% respectively. There was a significant difference between group B, C and D and group A (P <0.01) There was a significant difference between group B, group D and group B (P <0.01), but there was no significant difference between group D and group C (P> 0.05). With the increase of particle dose, the proportion of G2 / M phase cells increased, with significant difference between the groups (P <0.01). 125I particles can induce apoptosis of human esophageal cancer Eca-109 cells cultured in vitro. The ability of 125I particles to induce apoptosis may be achieved by arresting cells at G2 / M phase.