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目的:研究不同年龄段脑出血患者的载脂蛋白E(ApoE)基因多态性及其与血脂水平的关系。方法:选择2017年12月至2019年12月威海市中心医院神经外科接收的脑出血住院患者360例为脑出血组，根据年龄将脑出血患者分为低年龄组(年龄60岁)，每组患者120例；另选择同期在该院接受健康体检且结果为健康者120例为对照组。检测外周血中高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、甘油三酯(TG)、总胆固醇(TC)水平；采用聚合酶链式反应一限制性片段长度多态性方法测定ApoE基因多态性。结果:脑出血组患者性别、年龄、体质量指数与对照组差异无统计学意义(n t/χn 2＝0.045、0.459、0.655，n P＝0.832、0.323、0.256)；脑出血组患者收缩压、舒张压、高血压史、糖尿病史、吸烟史以及饮酒史均显著高于对照组(n t/χn 2＝12.598、13.003、49.308、10.246、45.562、8.602，n P＝0.000、0.000、0.000、0.000；0.000、0.003)；对照组、低龄组、中龄组和高龄组患者TC、TG、LDL和HDL之间差异均有统计学意义(n F＝36.893、131.263、808.908、143.188，均n P＝0.000)；对照组、低龄组、中龄组和高龄组最高的基因型频率是E3/3，分别为70.00%、68.33%、68.33%、66.67%，各组ApoE的E2/2、E2/3、E2/4、E3/3、E3/4和E4/4基因型频率差异无统计学意义(χn 2＝2.025、1.524、0.693、0.308、0.815、2.025，n P＝0.567、0.677、0.875、0.959、0.856、0.567)；对照组、低龄组、中龄组和高龄组最高等位基因频率为ε3，分别为81.66%、81.66%、79.99%、80.83%，各组ApoE的基等位基因的频率差异无统计学意义(χn 2＝0.061、0.125、0.241，n P＝0.996、0.989、0.971)；对于E3/3基因型脑出血患者，低龄组TC、TG、LDL和HDL分别为(4.43±0.33)mmol/L、(1.50±0.37)mmol/L、(2.58±0.32)mmol/L和(1.53±0.32)mmol/L，中龄组TC、TG、LDL和HDL分别为(4.22±0.34)mmol/L、(1.87±0.32)mmol/L、(3.30±0.31)mmol/L和(1.85±0.35)mmol/L，高龄组TC、TG、LDL和HDL分别为(3.85±0.35)mmol/L、(2.09±0.35)mmol/L、(3.60±0.33)mmol/L和(2.13±0.34)mmol/L，各组患者间的TC、TG、LDL和HDL差异均有统计学意义(n F＝60.289、59.844、218.120、64.364，均n P＝0.000)。n 结论:不同年龄段脑出血患者ApoE基因的多态性与广泛意义上脑出血的发生之间无显著的相关性，不同年龄段脑出血患者E3/3基因型与血脂水平之间无相关性。“,”Objective:To study ApoE gene polymorphism in patients with intracerebral hemorrhage at different ages and its relationship with blood lipid level.Methods:From December 2017 to December 2019, 360 inpatients with cerebral hemorrhage received by neurosurgery department of Weihai Central Hospital were selected as the cerebral hemorrhage group.According to the age of patients, patients with cerebral hemorrhage were divided into low age group (age60 years), with 120 cases in each group.Another 120 healthy persons who received health examination during the same period were selected as control group.The levels of high density lipoprotein (HDL), low density lipoprotein (LDL), triglyceride (TG) and total cholesterol (TC) in peripheral blood were measured.ApoE gene polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results:There were no statistically significant differences in sex, age, body mass index between the cerebral hemorrhage group and the control group (χn 2=0.045, n t=0.459, n t=0.655, n P=0.832, 0.323, 0.256). The systolic blood pressure, diastolic blood pressure, history of hypertension, history of diabetes, smoking and drinking in the cerebral hemorrhage group were significantly higher than those in the control group (n t=12.598, n t=13.003, χ n 2=49.308, χ n 2=10.246, χ n 2=45.562, χ n 2=8.602, n P=0.000, 0.000, 0.000, 0.000, 0.000, 0.003). There were statistically significant differences in TC, TG, LDL and HDL among the control group, the younger group, the middle-aged group and the older group (n F=36.893, 131.263, 808.908, 143.188, all n P=0.000). The highest genotype frequency was E3/3 in the control group, the younger group, the middle-aged group and the older group(70.00%, 68.33%, 68.33% and 66.67%). There was no statistically significant difference in genotype frequencies of ApoE among the groups (χn 2=2.025, 1.524, 0.693, 0.308, 0.815, 2.025, n P=0.567, 0.677, 0.875, 0.959, 0.856, 0.567). The highest allele frequencies of ApoE in the control group, the younger group, the middle-aged group and the older group were epsilon 3 (81.66%, 81.66%, 79.99%, 80.83%). There was no statistically significant difference in the allele frequencies of ApoE among the groups (χn 2=0.061, 0.125, 0.241, n P=0.996, 0.989, 0.971). For patients with E3/3 genotype cerebral hemorrhage, the levels of TC, TG, LDL and HDL in the younger age group were (4.43±0.33)mmol/L, (1.50±0.37)mmol/L, (2.58±0.32)mmol/L and (1.53±0.32)mmol/L, respectively.The TC, TG, LDL and HDL levels in middle-aged group were (4.22±0.34)mmol/L, (1.87±0.32)mmol/L, (3.30±0.31)mmol/L and (1.85±0.35)mmol/L, respectively.The TC, TG, LDL and HDL levels in the elderly group were (3.85±0.35)mmol/L, (2.09±0.35)mmol/L, (3.60±0.33)mmol/L and (2.13±0.34)mmol/L, respectively.There were statistically significant differences in TC, TG, LDL and HDL among all groups (n F=60.289, 59.844, 218.120, 64.364, all n P=0.000).n Conclusion:There is no significant correlation between ApoE gene polymorphism and the occurrence of widespread intracerebral hemorrhage in different age groups.There is no correlation between E3/3 genotype and blood lipid level in patients with intracerebral hemorrhage in different age groups.