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目的:应用大黄和酚酞建立一种大鼠“泻剂结肠”的动物模型。方法:健康成年Wistar大鼠84只,分2期,第1期36只,第2期48只,均分为对照组(饲以普通饲料),大黄组(饲料中添加有大黄粉)和酚酞组(饲料中添加酚酞)。第1期:大黄及酚酞开始剂量为100mg/kg·d~(-1),逐渐递增,30d后,大黄剂量为3 200mg/kg·d~(-1),酚酞为4000mg/kg·d~(-1);第2期:大黄及酚酞起始剂量为200mg/kg·d~(-1),3个月后,大黄最终用量为2600rag/kg·d~(-1),酚酞为3600rog/kg·d~(-1)。活性炭悬液推进法测定肠道传输功能,并行结肠标本HE染色及肌间神经丛嗜银染色。结果:与对照组相比,两期试验中大黄组和酚酞组肠道传输均有明显减慢;肌间神经丛嗜银染色大黄组和酚酞组有肌间神经丛嗜银性减弱甚至消失。结论:本“泻剂结肠”大鼠模型具有慢传输性便秘的肠道传输延迟和肌间神经丛嗜银性明显降低等肠道功能及病理变化,简单经济,可重复性强。
Objective: To establish a rat model of “cathartic colon” using rhubarb and phenolphthalein. Methods: A total of 84 healthy adult Wistar rats were divided into two phases, the first phase was 36, the second phase was 48, divided into control group (fed with normal diet), rhubarb group (feed added with rhubarb powder) and phenolphthalein Group (feed phenolphthalein). The initial dose of rhubarb and phenolphthalein was 100mg / kg · d ~ (-1), and gradually increased. After 30 days, the dosage of rhubarb was 3 200mg / kg · d ~ (-1) and the concentration of phenolphthalein was 4000mg / kg · d ~ (-1); No.2: The initial dose of rhubarb and phenolphthalein was 200mg / kg · d -1. After 3 months, the final dosage of rhubarb was 2600rag / kg · d -1, phenolphthalein was 3600rog / kg · d ~ (-1). Activated charcoal suspension method for determination of intestinal transit function, parallel colonic HE staining and myenteric plexus silver staining. RESULTS: Compared with the control group, both the rhubarb group and the phenolphthalein group significantly decreased the intestinal transit in both groups. The myelination of the myenteric plexus of the rhubarb group and the phenolphthalein group in the myelinated plexus group was weakened or even disappeared. CONCLUSION: The rat model of “cathartic colon” has slow transit intestinal transit delay and decreased myelophilic argyrophilicity such as slow transit constipation. The simple and economical, reproducible and reproducible intestinal function and pathological changes were observed.