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目的 探讨G CSF和IL 6联合应用协同促进受辐射小鼠造血功能恢复的作用及机理。方法 采用F 80 0血细胞自动计数仪计数红细胞、白细胞、血小板和血红蛋白等指标。常规法进行骨髓集落培养、内源性脾结节计数。琼脂糖电泳方法检测骨髓细胞程序性死亡。结果 G CSF和IL 6联合应用促进受辐射小鼠外周血细胞的恢复 ,促进脾脏内源性脾结节的形成 ,增加骨髓细胞含量和促进骨髓细胞GM CFU的形成 ,提高受辐射小鼠的生存率。结论 G CSF和IL 6联合应用的效果优于单用的效果 ,其促进辐射后机体造血功能恢复的机理是通过刺激骨髓残余造血干细胞的恢复 ,该过程需要一段较长的时间 ,而不是通过抑制照后即刻骨髓细胞的程序性死亡
Objective To investigate the synergistic effect of G CSF and IL 6 on the recovery of hematopoietic function in irradiated mice. Methods Red blood cell, white blood cell, platelet and hemoglobin were counted by F 80 O blood cell counting device. Routine method for bone marrow colony culture, endogenous spleen tuber count. Agarose electrophoresis was used to detect apoptosis in bone marrow cells. Results The combination of G CSF and IL 6 promoted the recovery of peripheral blood cells in irradiated mice, promoted the formation of spleen endogenous splenic nodules, increased the content of bone marrow cells and promoted the formation of GM CFU in bone marrow cells, and improved the survival rate of irradiated mice. . Conclusion The combined effect of G CSF and IL 6 is superior to that of single use. The mechanism of promoting hematopoietic recovery after radiation is through stimulating the recovery of residual hematopoietic stem cells in the bone marrow. This process takes a long time, not through inhibition. Programmed death of bone marrow cells immediately after exposure