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目的研究双酚A(bisphenol A,BPA)对C57BL/6雌性小鼠脾细胞线粒体膜电位及氧化应激的影响。方法将24只4~5周龄清洁级C57BL/6雌性小鼠按体重随机分为3组,分别为对照(水中无水乙醇的终浓度为1%)组和1、10μg/ml BPA暴露组,每组8只。采用饮水方式进行染毒,连续1个月。检测小鼠脾细胞线粒体膜电位、雌激素受体(ERα)蛋白表达水平及丙二醛(MDA)、超氧化物歧化酶(SOD)和总抗氧化能力(T-AOC)水平。结果与对照组比较,各剂量BPA暴露组小鼠脾细胞线粒体膜电位及SOD和T-AOC的水平均降低,而10μg/ml BPA暴露组小鼠脾细胞MDA水平较高,差异均有统计学意义(P<0.05);而各剂量BPA暴露组小鼠脾细胞ERα蛋白的表达水平均没有明显改变。结论在本实验剂量下,BPA暴露可引起C57BL/6雌性小鼠脾细胞凋亡,其机制与BPA诱导的氧化损伤有关;BPA对小鼠脾细胞的毒性作用可能与ERα信号通路无关。
Objective To investigate the effects of bisphenol A (BPA) on mitochondrial membrane potential and oxidative stress in splenocytes of C57BL / 6 female mice. Methods Twenty-four clean-grade C57BL / 6 female mice aged 4-5 weeks were randomly divided into three groups according to body weight: control (water-free ethanol concentration of 1%) and 1,10μg / ml BPA exposure group , Each group of 8. Adopt the way of drinking water for 1 month in a row. The mitochondrial membrane potential, estrogen receptor (ERα) protein expression and the levels of malondialdehyde (MDA), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) were detected. Results Compared with the control group, the levels of mitochondrial membrane potential and the levels of SOD and T-AOC in spleen cells of mice exposed to BPA at all doses were decreased, while the levels of MDA in spleen cells of 10 μg / ml BPA-exposed mice were significantly higher than those of the control group (P <0.05). However, there was no significant change in the expression levels of ERαprotein in the splenocytes of BPA-exposed mice. Conclusions BPA exposure can induce the apoptosis of splenocytes in C57BL / 6 female mice. The mechanism of BPA exposure is related to BPA-induced oxidative damage. The cytotoxicity of BPA to mouse spleen cells may not be related to the ERα signaling pathway.