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笔者以正常人外周血淋巴细胞为实验材料，加入２ μｇ／ｍｌ 的ＰＨＡ刺激，用３Ｈ—ＴｄＲ标记，通过放射自显影，测定培养不同时间后进入细胞周期的淋巴细胞数量及Ｍ 期的细胞数量，探讨细胞内动粒蛋白含量变化与淋巴细胞增殖的关系。ＡＣＡ间接免疫荧光染色显示，正常未经转化的淋巴细胞和加ＰＨＡ刺激培养至４８ ｈ 的淋巴细胞内动粒荧光斑点小而微弱，加ＰＨＡ刺激培养５４～７２ ｈ 的淋巴细胞内动粒荧光斑点亮度逐渐增强，体积逐渐增大。Ｗｅｓｔｅｒｎ ｂｌｏｔ 表明，１７ＫＤ和５２ＫＤ两种动粒蛋白在对照组和培养不同时间的各组淋巴细胞内均存在，且含量保持相对稳定，８０ＫＤ和５６ＫＤ两种动粒蛋白在未经培养和培养至４８ ｈ 的淋巴细胞内不出现，８０ＫＤ蛋白在培养５４ ｈ 的细胞内可见。培养６０～７２ ｈ，淋巴细胞内可被ＡＣＡ 特异识别的动粒蛋白共四种：８０ＫＤ、５６ＫＤ、５２ＫＤ和１７ＫＤ。
The author of normal peripheral blood lymphocytes as experimental materials, adding 2 μg / ml of PHA stimulation, 3H-TdR labeling, by autoradiography, measured at different times after the culture into the cell cycle of lymphocytes and the number of M cells To investigate the relationship between the changes of intracellular actin and lymphocyte proliferation. Indirect immunofluorescence staining of ACA showed that within 48 h of normal non-transformed lymphocytes and PHA-stimulated lymphocytes cultured within 48 h of dynamic small and weak spot fluorescence particles, plus PHA stimulated 54-72 h cultured lymphocytes within the dynamic particle spot Brightness gradually increased, the volume gradually increased. Western blot showed that 17KD and 52KD two kinds of kinetochore protein in control group and cultured at different times in each group of lymphocytes exist, and the content remained relatively stable, 80KD and 56KD two kinds of kinetochore protein in cultured and cultured to 48 h in lymphocytes did not appear, 80KD protein in cultured cells can be seen within 54 h. Cultured 60 ~ 72 h, lymphocytes can be ACA-specific recognition of the four kinds of kinetochore proteins: 80KD, 56KD, 52KD and 17KD.