目的:建立在小鼠心肌细胞中特异性过表达Hole转基因小鼠。方法:构建心肌特异性过表达Hole的转基因载体,显微注射导入小鼠受精卵,通过胚胎移植,获得转基因首建者小鼠。利用PCR检测Hole基因整合情况,并通过实时定量PCR检测Hole基因过表达效率。结果:PCR检测发现有6只小鼠在其基因组上整合有Hole载体基因。实时定量PCR结果显示,在心脏组织中Hole基因的转录本表达明显升高。结论:成功建立了在小鼠心肌细胞中特异性过表达Hole转基因小鼠,为研究Hole基因在心脏发育与相关疾病中的功能及机制提供了动物模型。 OBJECTIVE: To establish a mouse model of overexpression of Hole transgenic mice in mouse cardiomyocytes. Methods: We constructed a gene-specific vector expressing Hole that was overexpressed in Myocardium, and injected the mouse zygotes into mice by microinjection. Transgenic mice were obtained by embryo transfer. Hole gene integration was detected by PCR and Hole gene overexpression efficiency was detected by real-time quantitative PCR. RESULTS: Sixty-six mice were found by PCR to integrate the Hole vector into their genome. Real-time quantitative PCR results showed that the expression of Hole gene in heart tissue was significantly increased. CONCLUSIONS: Hole transgenic mice specifically overexpressed in mouse cardiomyocytes were successfully established and provided an animal model for studying the function and mechanism of Hole gene in cardiac development and related diseases.