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目的 :检测体外培养的人口腔黏膜上皮异常增生细胞 (DysplasticOralKeritinocyte ,DOK ) ,为口腔癌前病变体外细胞模型建立提供试验依据。方法 :采用细胞计数 ,生长曲线 ,克隆形成率 ,染色体倍性检测等方法 ,比较DOK与正常口腔黏膜上皮角化细胞 (OralKeritinocytes ,OKC)及Tca8113 (舌鳞癌细胞 )细胞体外培养的生物学特性。结果 :DOK在生长速度 ,克隆形成率 ,染色体异倍体率均介于正常细胞与肿瘤细胞之间。结论 :DOK细胞属正常细胞向癌转变的中间过渡型细胞 ,可作为研究口腔癌前病变的细胞模型。
OBJECTIVE: To detect human oral epithelial dysplasia (DOK) cells cultured in vitro to provide experimental evidence for the establishment of oral precancerous lesions in vitro. Methods: The biological characteristics of DOK and normal oral mucosa epithelial cells (OKC) and Tca8113 (tongue squamous cell carcinoma) cells in vitro were compared by cell counting, growth curve, clone formation rate and chromosome ploidy detection. . Results: The growth rate of DOK, the rate of colony formation and the rate of chromosome aneuploidy were all between normal cells and tumor cells. CONCLUSION: DOK cells are intermediate transitional cells transformed from normal cells to carcinomas, and can be used as a model for the study of precancerous lesions in oral cavity.