Hepatotropic growth factors protect hepatocytes during inflammation by upregulation of antioxidative

来源 :World Journal of Gastroenterology | 被引量 : 0次 | 上传用户:tanhuafuren
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
AIM:To investigate effects of hepatotropic growth factors on radical production in rat hepatocytes during sepsis.METHODS:Rat hepatocytes,isolated by collagenase perfusion,were incubated with a lipopolysaccharide(LPS)-containing cytokine mixture of interleukin-1β,tumor necrosis factor-α and interferon-γ to simulate sepsis and either co-incubated or pre-incubated with hepatotropic growth factors,e.g.hepatocyte growth factor,epidermal growth factor and/or transforming growth factor-α.Cells were analyzed for glutathione levels.Culture supernatants were assayed for produc-tion of reactive oxygen intermediates(ROIs) as well as NO2-,NO3-and S-nitrosothiols.To determine cellular damage,release of aspartate aminotransferase(AST) into the culture medium was analyzed.Activation of nuclear factor(NF)-κB was measured by electrophoretic mobility shift assay.RESULTS:Rat hepatocytes treated with the LPS-containing cytokine mixture showed a significant increase in ROI and nitrogen oxide intermediate formation.AST leakage was not significantly increased in cells treated with the LPS-containing cytokine mixture,independent of growth-factor co-stimulation.However,pretreatment with growth factors significantly reduced AST leakage and ROI formation while increasing cellular glutathione.Application of growth factors did not result in increased NF-κB activation.Pretreatment with growth factors further increased formation of NO2-,NO3-and S-nitrosothiols in hepatocytes stimulated with LPS-containing cytokine mixture.Thus,we propose that,together with an increase in glutathione increased NO2-,NO3-formation might shift their metabolism towards non-toxic products.CONCLUSION:Our data suggest that hepatotropic growth factors positively influence sepsis-induced hepatocellular injury by reducing cytotoxic ROI formation via induction of the cellular protective antioxidative systems. AIM: To investigate effects of hepatotropic growth factors on radical production in rat hepatocytes during sepsis. METHODS: Rat hepatocytes, isolated by collagenase perfusion, were incubated with a lipopolysaccharide (LPS) -containing cytokine mixture of interleukin-1β, tumor necrosis factor-α and interferon-γ to simulate sepsis and either co-incubated or pre-incubated with hepatotropic growth factors, eg hepatocyte growth factor, epidermal growth factor and / or transforming growth factor-α. Cells were analyzed for glutathione levels. Culture supernatants were assayed for produc-tion of reactive oxygen intermediates (ROIs) as well as NO2-, NO3- and S- nitrosothiols. To determine cellular damage, release of aspartate aminotransferase (AST) into the culture medium was analyzed. Activation of nuclear factor (NF) κB was measured by electrophoretic mobility shift assay .RESULTS: Rat hepatocytes treated with the LPS-containing cytokine mixture showed a significant increase in ROI and nitrogen oxide intermedia te formation. AST leakage was not significantly increased in cells treated with the LPS-containing cytokine mixture, independent of growth-factor co-stimulation. However, pretreatment with growth factors significantly reduced AST leakage and ROI formation while increasing cellular glutathione. Application of growth Factors did not result in increased NF-κB activation. Treatment with growth factors further increased formation of NO2-, NO3-and S-nitrosothiols in hepatocytes stimulated with LPS-containing cytokine mixture .hus, we propose that, together with an increase in glutathione increased NO2-, NO3-formation might shift their metabolism towards non-toxic products. CONCLUSION: Our data suggest that hepatotropic growth factors positively influenced sepsis-induced hepatocellular injury by reducing cytotoxic ROI formation via induction of the cellular protective antioxidative systems.
其他文献
前不久,武汉雅琪等相继宣布退出足坛,将股份部分转让。万宝路集团宣布将不再赞助甲A联赛,使一向颇受投资者青睐的足球一下子变成了烫手的山芋,于是,有人担心会引发一场足坛经济危机
高仲龙 ,男 ,汉族 ,辽宁省沈阳市人 ,1 932年 8月出生 ,中共党员。 1 953年东北工学院轧钢专业本科毕业 ,1 955年东北工学院冶金炉专业研究生毕业。现任北京科技大学热能工程
1965年,在东北工学院的协助下,我们研制了单层罐笼楔型安全卡,经多年使用效果良好。又于1974~1979年,从华铜矿引进双层罐笼楔型安全卡,并结合我矿情况进行改制,从而在四个主
本文研究了大的棕色蛰伏蝙蝠在体温为5、10、20、30及37℃时对低氧及高碳酸血症的通气反应及通气模式。结果发现,体温低于30℃时,产生同歇性呼吸模式,表现为节律性呼吸发放
我厂自1980午开始,在减速机中使用二硫化钼齿轮专用脂挤压成膜法,取得了很好的效果,节约了润滑油、解决了漏油问题、消灭了设备润滑事故。这种齿轮专用脂由天津汉沽石油化学
研究了普通镀钴溶液中碳化硅微粒与基质金属钴形成复合镀层的电沉积过程.结果表明,在小的微粒载荷量下,复合电沉积过程由微粒向阴极的传输所控制.而在大的微粒载荷量下,小电流密度
§1 概述在工业控制及国防工业中,广泛地应用了数字计算机,因而使模拟数字转换装置就成为不可缺少的设备了。本文仅就模拟数字转换装置的一种——接触式“V 形编码器”加以
介绍了一种通过计算分子的同位素丰度模拟质谱峰型,根据比较质谱峰的位置和形状来帮助归属多元素团簇的飞行时间质谱的方法。这种方法不仅有助于归属完全分辨的低质量离子峰,还
我公司在加工如图1所示的工件时,采用图2所示的夹具进行加工,通过转动螺杆2,推动夹紧块3进行夹紧。由于该工件的外形 My company in the processing of the workpiece shown
本期封面人物:王庆堂,南安籍香港企业家,1973年赴香港定居后,一直从事再生棉纺织行业。1987年回大陆投资,在深圳观澜创办了深圳第一个再生棉厂,到1992年发展到了3个厂,年产量达到400