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目的检测男性泌尿生殖道分离奈瑟菌的16SrDNA和PIA基因,探讨奈瑟菌属菌种的基因鉴定及其致病机理。方法用PCR扩增和核苷酸序列分析方法分别检测11例男性泌尿生殖道感染患者泌尿生殖道分离的14株奈瑟菌属菌种的16SrDNA和PIA基因及其序列。结果 14株奈瑟菌属菌种经16SrDNA检测鉴定分别为淋病奈瑟菌2株,黏液奈瑟菌3株,灰色奈瑟菌5株,微黄奈瑟菌2株,干燥奈瑟菌1株,嗜乳糖奈瑟菌及多糖奈瑟菌各1株;与常规细菌学方法鉴定的符合率为85.7%。非淋球菌奈瑟菌未检出淋病奈瑟菌毒力相关的PIA核苷酸序列。结论常规细菌学方法与染色体16SrDNA检测及其序列分析方法的联合使用,可提高奈瑟菌属菌种感染的实验室诊断准确率;PIA基因对于奈瑟菌属的男性生殖道致病性无关。
Objective To detect 16S rDNA and PIA genes of Neisseria isolates from male genitourinary tract and investigate the genetic identification and pathogenic mechanism of Neisseria species. Methods The 16S rDNA and PIA genes of 14 strains of Neisseria isolated from urogenital tract of 11 male genitourinary tract infections were detected by PCR and nucleotide sequence analysis. Results 14 strains of Neisseria were identified as 16 strains of Neisseria gonorrhoeae, 3 strains of Neisseria gonorrhoeae, 5 strains of Neisseria gonorrhoeae, 2 strains of Neisseria gonorrhoeae, 2 strains of Neisseria gonorrhoeae, 1 strain of Neisseria gonorrhoeae , Neisseria lactam and one polysaccharide Neisseria gonorrhoeae respectively. The coincidence rate with conventional bacteriological method was 85.7%. Neisseria gonorrhoeae Neisseria gonorrhoeae virulence-associated PIA nucleotide sequence was not detected. Conclusion The combined use of routine bacteriological methods and chromosome 16S rDNA detection and sequence analysis can improve the accuracy of laboratory diagnosis of Neisseria infection. PIA gene has no relation with pathogenicity of Neisseria in male genital tract.