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目的:探讨红旱莲主要有效成分黄酮类化合物的提取工艺。方法:采用正交试验法,以总黄酮、金丝桃苷的含量为评价指标,以乙醇浓度、溶媒用量、提取时间、提取次数为考察因素,对红旱莲乙醇回流提取工艺进行优化。结果:最佳提取工艺为:10倍量80%乙醇,回流提取2次,每次3h。结论:本实验设计合理,测定方法可行,实验结果可靠。
Objective: To investigate the extraction of flavonoids from the main active constituents of Eclipta. Methods: The orthogonal test was used to evaluate the content of total flavonoids and hyperoside. The ethanol concentration, the amount of solvent, the extraction time and the number of extraction were taken as the investigation factors to optimize the ethanol reflux extraction process. Results: The best extraction process: 10 times the amount of 80% ethanol, reflux extraction 2 times, each time 3h. Conclusion: The experimental design is reasonable, the determination method is feasible and the experimental results are reliable.