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根据Ｅ．ｃｏｌｉ基因组ｒＤＮＡ保守序列设计的两对引物，分别用于扩增３０株来源于我国不同地区的快生型大豆根瘤菌（Ｓｉｎｏｒｈｉｚｏｂｉｕｍｓｐｐ）菌株。扩增产物经几种较少识别序列的内切酶消化后，得出特征的限制性内切酶酶切图谱（ＲＦＬＰ）。该图谱可用于供试菌株种水平的鉴定及菌株ｒＤＮＡ分子的遗传多样性研究。选用来源于Ｅ．ｃｏｌｉ基因组１６ＳｒＤＮＡ保守序列的一对引物（ＰＨ和ＰＡ）将所有供试菌株都扩增出一条１．５ｋｂ的带，经四种内切酶消化后发现：新疆地区来源的菌株的酶切图谱显著不同于其它地区来源的菌，称其为群Ⅰ菌株。群Ⅰ菌株内部的酶切图谱还可以分为５种类型。其它地区来源菌株的酶切图谱基本一致（７５％），称其为群Ⅱ菌株。在群Ⅱ菌株内部，有少数菌株也表现有特殊性。选用来源于Ｅ．ｃｏｌｉ基因组的１６ＳｒＤＮＡ和２３ＳｒＤＮＡ保守序列的一对引物ＰＨＲ和Ｐ２３ＳＲＯ１将新疆来源的一群菌株扩增出一条１．５ｋｂ的带，而其它地区来源的菌株扩增产物为１．８ｋｂ。新疆来源的群Ｉ菌株扩增产物经３种内切酶消化后的ＲＦＬＰ图谱显著不同于群Ⅱ菌株。群Ⅰ菌株的ＲＦＬＰ图谱也不完全一致，表现为４种类型。群Ⅱ菌株ＲＦＬＰ图谱基本一致，但也发现有特?
According to E. Two pairs of primers designed by the conserved sequence of rDNA in the genome of E. coli were used to amplify 30 strains of fast-growing strains of Sinorhizobium spp from different regions in China. Restriction endonuclease digestion pattern (RFLP) was obtained after amplification products were digested with several less recognition sequences. The map can be used for the identification of strain level and the genetic diversity of the rDNA molecule of the strain. Choose from E. A pair of primers (PH and PA) of the conserved sequence of 16SrDNA in E. coli genome amplified a 1.5kb band in all the tested strains. After digestion with four kinds of endonucleases, it was found that the restriction map of strains from Xinjiang origin was significant Unlike other sources of bacteria, called Group I strains. Group Ⅰ strains within the restriction map can also be divided into five types. Strains from other regions of the enzyme digestion map is basically the same (75%), called Group Ⅱ strains. Within the group Ⅱ strains, there are a few strains also showed particularity. Choose from E. A pair of primers PHR and P23SRO1 of 16SrDNA and 23SrDNA conserved sequences in the genome of E. coli were used to amplify a 1.5kb band from a group of Xinjiang-derived strains and 1.8kb from strains from other regions. The RFLP patterns of the population of swine I strain amplified from Xinjiang were significantly different from those of group II after digested by three kinds of endonucleases. The group Ⅰ strain RFLP maps are not exactly the same, showing four types. Group Ⅱ strain RFLP basically the same atlas, but also found that special?