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本文应用镧电子示踪技术和焦锑酸分子探针技术及体视学定量方法,观察了心肌缺血不同时间再灌注后细胞膜系和线粒体镧(La~(3+))、钙(Ca~(2+))示踪所提示的线粒体的功能以及超微结构变化等特点。结果表明,心肌缺血/再灌注反应具有一定规律性,即反应初期—反应极期—反应表竭期。缺血40min到60min再灌注是可逆性与不可逆性再灌注损伤的临界点。
In this paper, the effects of lanthanum (La ~ (3+)) and calcium (Ca ~ (2+)) on cell membrane and mitochondria after myocardial ischemia at different time points were observed using lanthanum electron tracing and pyrogallol molecular probe techniques and stereological methods. (2+) tracing the suggested mitochondrial function and ultrastructural changes. The results showed that myocardial ischemia / reperfusion reaction has a certain regularity, that is, the initial reaction - the reaction period - the reaction of the table. Reperfusion after 40min to 60min of ischemia is the critical point of reversible and irreversible reperfusion injury.